Multiple cloning site (MCS)
TurboFP650	BspE I				Xho I				Hind III			Pst I					Kpn I			Apa I				BamH I					STOP
				Bgl II			Sac I				EcoR I			Sal I				Sac II				Sma I/Xma I						Xba I#
...	TCC.GGA	.CTC	.	AGA.T	CT	.C	GA.G	CT.C	AA.GCT.T	C	G.AAT.T	C	T.GCA.	G	TC.GAC	.	GGT.A	CC	.GC	G.G	G	C.CC	G.	GG	A.TCC	.ACC.GGA	.	TC	T.AG	A	....

# – sites are blocked by dam methylation. If you wish to digest the vector with these enzymes, you will need to transform the vector into a dam^- host and make fresh DNA.