Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
MCS: 591-671
TurboFP650
Kozak consensus translation initiation site: 672-682
Start codon (ATG): 679-681
Stop codon: 1381-1383
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1536-1541 & 1565-1570
mRNA 3' ends: 1574 & 1586
f1 single-strand DNA origin: 1633-2088
Bacterial promoter for expression of Kan^r gene
-35 region: 2150-2155
-10 region: 2173-2178
Transcription start point: 2185
SV40 origin of replication: 2429-2564
SV40 early promoter
Enhancer (72-bp tandem repeats): 2262-2333 & 2334-2405
21-bp repeats: 2409-2429, 2430-2450 & 2452-2472
Early promoter element: 2485-2491
Major transcription start points: 2481, 2519, 2525 & 2530
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2613-2615
Stop codon: 3405-3407
G->A mutation to remove Pst I site: 2795
C->A (Arg to Ser) mutation to remove BssH II site: 3141
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3643-3648 & 3656-3661
pUC plasmid replication origin: 3992-4635