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    pTurboGFP-dest1

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pTurboGFP-dest1 vector

cat.# FP519

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

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vector information:
ProductCat.#SizePrice
pTurboGFP-dest1FP51920 μg€ 400.00
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Vector typemammalian expression vector
ReporterTurboGFP
Reporter codon usagemammalian
Promoter for TurboGFPPCMV IE
Host cellsmammalian
Selectionprokaryotic - kanamycin
eukaryotic - neomycin (G418)
Replicationprokaryotic - pUC ori
eukaryotic - SV40 ori
Use TurboGFP expression in mammalian cells; generation of fusions to the TurboGFP-dest1 N-terminus

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
MCS: 591-671
TurboGFP
Kozak consensus translation initiation site: 672-682
Start codon (ATG): 679-681
Last amino acid in TurboGFP: 1372-1374
Stop codon: 1510-1512
MODC PEST sequence: 1390-1512
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1667-1672 & 1696-1701
mRNA 3' ends: 1705 & 1717
f1 single-strand DNA origin: 1764-2219
Eukaryotic promoter for expression of Kanr gene
-35 region: 2281-2286
-10 region: 2304-2309
Transcription start point: 2316
SV40 origin of replication: 2560-2695
SV40 early promoter
Enhancer (72-bp tandem repeats): 2393-2464 & 2465-2536
21-bp repeats: 2540-2560, 2561-2581 & 2583-2603
Early promoter element: 2616-2622
Major transcription start points: 2612, 2650, 2656 & 2661
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2744-2746
Stop codon: 3536-3538
G->A mutation to remove Pst I site: 2926
C->A (Arg to Ser) mutation to remove BssH II site: 3272
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3774-3779 & 3787-3792
pUC plasmid replication origin: 4123-4766


Notice to Purchaser:

Evrogen Fluorescent Protein Products (the Products) are intended for research use only. The Products are covered by U.S. Pat. # 7,417,131 and other Evrogen Patents and/or Patent applications pending. By use of these Products, you accept the terms and conditions of the applicable Limited Use Label License.

The CMV promoter is covered under U.S. Patents 5,168,062 and 5,385,839, and its use is permitted for research purposes only. Any other use of the CMV promoter requires a license from the University of Iowa Research Foundation, 214 Technology Innovation Center, Iowa City, IA 52242.

MATERIAL SAFETY DATA SHEET INFORMATION

To the best of our knowledge, these products do not require a Material Safety Data Sheet. However, all the properties of these products (and, if applicable, each of their components) have not been thoroughly investigated. Therefore, we recommend that you use gloves and eye protection, and wear a laboratory coat when working with these products.

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