Location of features

MCS: 12-96
TurboRFP
Kozak consensus translation initiation site: 90-100
Start codon (ATG): 97-99
Stop codon: 790-792
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 946-951 & 975-980
mRNA 3' ends: 984 & 996
f1 single-strand DNA origin: 1043-1498
Bacterial promoter for expression of Kan^r gene
-35 region: 1560-1565
-10 region: 1583-1588
Transcription start point: 1595
SV40 origin of replication: 1839-1974
SV40 early promoter
Enhancer (72-bp tandem repeats): 1672-1743 & 1744-1815
21-bp repeats: 1819-1839, 1840-1860 & 1862-1882
Early promoter element: 1895-1901
Major transcription start points: 1891, 1929, 1935 & 1940
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2023-2025
Stop codon: 2815-2817
G->A mutation to remove Pst I site: 2205
C->A (Arg to Ser) mutation to remove BssH II site: 2551
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3053-3058 & 3066-3071
pUC plasmid replication origin: 3402-4045