Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
TurboRFP-mito fusion
Start codon (ATG): 597-599
Mitochondrial targeting sequence (MTS): 597-683
Start of TurboRFP coding sequence (ATG): 705-707
Stop codon (TGA): 1398
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1554-1559 & 1583-1588
mRNA 3' ends: 1592 & 1604
f1 single-strand DNA origin: 1651-2106
Bacterial promoter for expression of Kan^r gene
-35 region: 2168-2173
-10 region: 2191-2196
Transcription start point: 2203
SV40 origin of replication: 2447-2582
SV40 early promoter
Enhancer (72-bp tandem repeats): 2280-2351 & 2352-2423
21-bp repeats: 2427-2447, 2448-2468 & 2470-2490
Early promoter element: 2503-2509
Major transcription start points: 2499, 2537, 2543 & 2548
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2631-2633
Stop codon: 3423-3425
G->A mutation to remove Pst I site: 2813
C->A (Arg to Ser) mutation to remove BssH II site: 3159
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3661-3666 & 3674-3679
pUC plasmid replication origin: 4010-4653