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    pTurboYFP-C

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pTurboYFP-C vector

cat.# FP611

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

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vector information:
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pTurboYFP-CFP61120 μg€ 400.00
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Vector typemammalian expression vector
ReporterTurboYFP
Reporter codon usagemammalian
Promoter for TurboYFPPCMV IE
Host cellsmammalian
Selectionprokaryotic - kanamycin
eukaryotic - neomycin (G418)
Replicationprokaryotic - pUC ori
eukaryotic - SV40 ori
Use TurboYFP expression in mammalian cells; generation of fusions to the TurboYFP C-terminus

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
TurboYFP
Kozak consensus translation initiation site: 606-616
Start codon (ATG): 613-615
Stop codon: 1420-1422
Last amino acid in TurboYFP: 1339-1341
MCS: 1342-1419
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1562-1567 & 1591-1596
mRNA 3' ends: 1600 & 1612
f1 single-strand DNA origin: 1659-2114
Eukaryotic promoter for expression of Kanr gene
-35 region: 2176-2181
-10 region: 2199-2204
Transcription start point: 2211
SV40 origin of replication: 2455-2590
SV40 early promoter
Enhancer (72-bp tandem repeats): 2288-2359 & 2360-2431
21-bp repeats: 2435-2455, 2456-2476 & 2478-2498
Early promoter element: 2511-2517
Major transcription start points: 2507, 2545, 2551 & 2556
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2639-2641
Stop codon: 3431-3433
G->A mutation to remove Pst I site: 2821
C->A (Arg to Ser) mutation to remove BssH II site: 3167
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3669-3674 & 3682-3687
pUC plasmid replication origin: 4018-4661


Notice to Purchaser:

Evrogen Fluorescent Protein Products (the Products) are intended for research use only and covered by Evrogen Patents and/or Patent applications pending. By use of these products, you accept the terms and conditions of the applicable Limited Use Label License.

The CMV promoter is covered under U.S. Patents 5,168,062 and 5,385,839, and its use is permitted for research purposes only. Any other use of the CMV promoter requires a license from the University of Iowa Research Foundation, 214 Technology Innovation Center, Iowa City, IA 52242.

MATERIAL SAFETY DATA SHEET INFORMATION

To the best of our knowledge, these products do not require a Material Safety Data Sheet. However, all the properties of these products (and, if applicable, each of their components) have not been thoroughly investigated. Therefore, we recommend that you use gloves and eye protection, and wear a laboratory coat when working with these products.

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