Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
MCS: 592-678
TurboYFP
Kozak consensus translation initiation site: 672-682
Start codon (ATG): 679-681
Stop codon: 1408-1410
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1564-1569 & 1593-1598
mRNA 3' ends: 1602 & 1614
f1 single-strand DNA origin: 1661-2116
Bacterial promoter for expression of Kan^r gene
-35 region: 2178-2183
-10 region: 2201-2206
Transcription start point: 2213
SV40 origin of replication: 2457-2592
SV40 early promoter
Enhancer (72-bp tandem repeats): 2290-2361 & 2362-2433
21-bp repeats: 2437-2457, 2458-2478 & 2480-2500
Early promoter element: 2513-2519
Major transcription start points: 2509, 2547, 2553 & 2558
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2641-2643
Stop codon: 3433-3435
G->A mutation to remove Pst I site: 2823
C->A (Arg to Ser) mutation to remove BssH II site: 3169
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3671-3676 & 3684-3689
pUC plasmid replication origin: 4020-4663