Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
MCS: 591-671
NirFP
Kozak consensus translation initiation site: 672-682
Start codon (ATG): 679-681
Stop codon: 1381-1383
Fragment of human beta globin (HBB) gene: 1392-2510
Last 35 bp of HBB exon 2: 1392-1426
HBB intron 2: 1427-2277
First 233 bp of HBB exon3: 2278-2510
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2652-2657 & 2681-2686
mRNA 3' ends: 2690 & 2702
f1 single-strand DNA origin: 2749-3204
Bacterial promoter for expression of Kan^r gene
-35 region: 3266-3271
-10 region: 3289-3294
Transcription start point: 3301
SV40 origin of replication: 3545-3680
SV40 early promoter
Enhancer (72-bp tandem repeats): 3378-3449 & 3450-3521
21-bp repeats: 3525-3545, 3546-3566 & 3568-3588
Early promoter element: 3601-3607
Major transcription start points: 3597, 3635, 3641 & 3646
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3729-3731
Stop codon: 4521-4523
G->A mutation to remove Pst I site: 3911
C->A (Arg to Ser) mutation to remove BssH II site: 4257
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4759-4764 & 4772-4777
pUC plasmid replication origin: 5108-5751