Multiple cloning site (MCS)
			Bgl II*			Sac I					EcoR I*			Sal I			Sac II				Sma I/Xma I				Age I			TurboGFP-dest1
	Afe I			Xho I*					Hind III			Pst I*				Kpn I			Apa I*				BamH I*				Nco I*
...	A.GCG.CT	A.CCG.GAC.TC	A.GAT.	CT	C.	GAG	.CTC	.	AAG.CTT	.C	GA.ATT.	C	TG.CA	G	.TCG.AC	G.GTA.	CC	G.C	GG	.G	CC.C	G	G.G	AT.CC	A.CCG.GT	C.GCC.A	CC.	ATG.G	...

* – not unique site.