Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
MCS: 591-671
TurboGFP-dest1
Kozak consensus translation initiation site: 672-682
Start codon (ATG): 679-681
Last amino acid in TurboGFP: 1372-1374
Amino acid residues of mouse ornithine decarboxylase (MODC) PEST sequence: 1390-1509
Stop codon: 1510-1512
Fragment of human beta globin (HBB) gene
Last 35 bp of HBB exon 2: 1521-1555
HBB intron 2: 1556-2406
First 233 bp of HBB exon 3: 2407-2639
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2781-2786 & 2810-2815
mRNA 3' ends: 2819 & 2831
f1 single-strand DNA origin: 2878-3333
Bacterial promoter for expression of Kan^r gene
-35 region: 3395-3400
-10 region: 3418-3423
Transcription start point: 3430
SV40 origin of replication: 3674-3809
SV40 early promoter
Enhancer (72-bp tandem repeats): 3507-3578 & 3579-3650
21-bp repeats: 3654-3674, 3675-3695 & 3697-3717
Early promoter element: 3730-3736
Major transcription start points: 3726, 3764, 3770 & 3775
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3858-3860
Stop codon: 4650-4652
G->A mutation to remove Pst I site: 4040
C->A (Arg to Ser) mutation to remove BssH II site: 4386
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4888-4893 & 4901-4906
pUC plasmid replication origin: 5237-5880