Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
mKate2-ER fusion: 597-1391
Start codon (ATG): 597-599
Calreticulin signal sequence: 597-647
mKate2: 663-1358
ER retention sequence (KDEL): 1380-1391
Stop codon: 1392-1394
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1593-1598 & 1622-1627
mRNA 3' ends: 1631 & 1643
f1 single-strand DNA origin: 1690-2145
Bacterial promoter for expression of Kan^r gene
-35 region: 2207-2212
-10 region: 2230-2235
Transcription start point: 2242
SV40 origin of replication: 2486-2621
SV40 early promoter
Enhancer (72-bp tandem repeats): 2319-2390 & 2391-2462
21-bp repeats: 2466-2486, 2487-2507 & 2509-2529
Early promoter element: 2542-2548
Major transcription start points: 2538, 2576, 2582 & 2587
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2670-2672
Stop codon: 3462-3464
G->A mutation to remove Pst I site: 2852
C->A (Arg to Ser) mutation to remove BssH II site: 3198
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3700-3705 & 3713-3718
pUC plasmid replication origin: 4049-4692