Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Kozak consensus translation initiation site: 606-616
mKate2
Start codon (ATG): 613-615
Last amino acid in mKate2: 1306-1308
Beta-Actin: 1342-2469
Stop codon: 2467-2469
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2630-2635 & 2659-2664
mRNA 3' ends: 2668 & 2680
f1 single-strand DNA origin: 2727-3182
Bacterial promoter for expression of Kan^r gene
-35 region: 3244-3249
-10 region: 3267-3272
Transcription start point: 3279
SV40 origin of replication: 3523-3658
SV40 early promoter
Enhancer (72-bp tandem repeats): 3356-3427 & 3428-3499
21-bp repeats: 3503-3523, 3524-3544 & 3546-3566
Early promoter element: 3579-3585
Major transcription start points: 3575, 3613, 3619 & 3624
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3707-3709
Stop codon: 4499-4501
G->A mutation to remove Pst I site: 3889
C->A (Arg to Ser) mutation to remove BssH II site: 4235
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4737-4742 & 4750-4755
pUC plasmid replication origin: 5086-5729