pmKate2-actinin vector

cat.# FP317

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pmKate2-actinin FP317 20 μg € 400 The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter mKate2 Reporter codon usage mammalian Promoter for mKate2 PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use far-red fluorescent labeling of α-actinin

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
actinin-mKate2 fusion: 637-4068
alpha-actinin: 637-3312
Start codon (ATG): 637-639
Last amino acid in alfa-Actinin: 3310-3312
mKate2: 3370-4068
Stop codon: 4066-4068
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 4221-4226 & 4250-4255
mRNA 3' ends: 4259 & 4271
f1 single-strand DNA origin: 4318-4773
Bacterial promoter for expression of Kan^r gene
-35 region: 4835-4840
-10 region: 4858-4863
Transcription start point: 4870
SV40 origin of replication: 5114-5249
SV40 early promoter
Enhancer (72-bp tandem repeats): 4947-5018 & 5019-5090
21-bp repeats: 5094-5114, 5115-5135 & 5137-5157
Early promoter element: 5170-5176
Major transcription start points: 5166, 5204, 5210 & 5215
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 5298-5300
Stop codon: 6090-6092
G->A mutation to remove Pst I site: 5480
C->A (Arg to Ser) mutation to remove BssH II site: 5826
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 6328-6333 & 6341-6346
pUC plasmid replication origin: 6677-7320