Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
mKate2-RhoB GTPase fusion
Start codon (ATG): 613-615
Start of mKate2 coding sequence (ATG): 613-615
Last amino acid in mKate2: 1312-1314
c-Myc epitope: 1357-1359
RhoB GTPase: 1383-1968
Stop codon: 1969-1971
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2163-2168 & 2192-2197
mRNA 3' ends: 2201 & 2213
f1 single-strand DNA origin: 2260-2715
Bacterial promoter for expression of Kan^r gene
-35 region: 2777-2782
-10 region: 2800-2805
Transcription start point: 2812
SV40 origin of replication: 3056-3191
SV40 early promoter
Enhancer (72-bp tandem repeats): 2889-2960 & 2961-3032
21-bp repeats: 3036-3056, 3057-3077 & 3079-3099
Early promoter element: 3112-3118
Major transcription start points: 3108, 3146, 3152 & 3157
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3240-3242
Stop codon: 4032-4034
G->A mutation to remove Pst I site: 3422
C->A (Arg to Ser) mutation to remove BssH II site: 3768
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4270-4275 & 4283-4288
pUC plasmid replication origin: 4619-5262