Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
mKate2-LAMP1 fusion
Start codon (ATG): 626-628
Lysosomal Associated Membrane Protein 1 (LAMP1) sequence: 626-1846
Start of mKate2 coding sequence (ATG): 1907-1909
Stop codon: 2603-2605
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2758-2763 & 2787-2792
mRNA 3' ends: 2796 & 2808
f1 single-strand DNA origin: 2855-3310
Bacterial promoter for expression of Kan^r gene
-35 region: 3372-3377
-10 region: 3395-3400
Transcription start point: 3407
SV40 origin of replication: 3651-3786
SV40 early promoter
Enhancer (72-bp tandem repeats): 3484-3555 & 3556-3627
21-bp repeats: 3631-3651, 3652-3672 & 3674-3694
Early promoter element: 3707-3713
Major transcription start points: 3703, 3741, 3747 & 3752
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3835-3837
Stop codon: 4627-4629
G->A mutation to remove Pst I site: 4017
C->A (Arg to Ser) mutation to remove BssH II site: 4363
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4865-4870 & 4878-4883
pUC plasmid replication origin: 5214-5857