Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
mKate2-PTS1 fusion
Start codon (ATG): 679-681
Start of mKate2 coding sequence (ATG): 679-681
Peroxisomal Targeting Signal 1 (PTS1): 1387-1395
Stop codon: 1396-1398
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1550-1555 & 1579-1584
mRNA 3' ends: 1588 & 1600
f1 single-strand DNA origin: 1647-2102
Bacterial promoter for expression of Kan^r gene
-35 region: 2164-2169
-10 region: 2187-2192
Transcription start point: 2199
SV40 origin of replication: 2443-2578
SV40 early promoter
Enhancer (72-bp tandem repeats): 2276-2347 & 2348-2419
21-bp repeats: 2423-2443, 2444-2464 & 2466-2486
Early promoter element: 2499-2505
Major transcription start points: 2495, 2533, 2539 & 2544
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2627-2629
Stop codon: 3419-3421
G->A mutation to remove Pst I site: 2809
C->A (Arg to Ser) mutation to remove BssH II site: 3155
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3657-3662 & 3670-3675
pUC plasmid replication origin: 4006-4649