RESEARCH PRODUCTS/Basic fluorescent proteins/TagFPs/mKate2

    pmKate2-vimentin

SEARCH

pmKate2-vimentin vector

cat.# FP318

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download
vector information:
ProductCat.#SizePrice
pmKate2-vimentinFP31820 μg€ 400
The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information.

Vector typemammalian expression vector
ReportermKate2
Reporter codon usagemammalian
Promoter for mKate2PCMV IE
Host cellsmammalian
Selectionprokaryotic – kanamycin
eukaryotic – neomycin (G418)
Replicationprokaryotic – pUC ori
eukaryotic – SV40 ori
Use far-red fluorescent labeling of vimentin

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Vimentin-mKate2 fusion: 637-2751
Vimentin: 637-2034
Start codon (ATG): 637-639
Last amino acid in Vimentin: 2032-2034
mKate2: 2056-2754
Stop codon: 2752-2754
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2907-2912 & 2936-2941
mRNA 3' ends: 2945 & 2957
f1 single-strand DNA origin: 3004-3459
Bacterial promoter for expression of Kanr gene
-35 region: 3521-3526
-10 region: 3544-3549
Transcription start point: 3556
SV40 origin of replication: 3800-3935
SV40 early promoter
Enhancer (72-bp tandem repeats): 3633-3704 & 3705-3776
21-bp repeats: 3780-3800, 3801-3821 & 3823-3843
Early promoter element: 3856-3862
Major transcription start points: 3852, 3890, 3896 & 3901
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3984-3986
Stop codon: 4776-4778
G->A mutation to remove Pst I site: 4166
C->A (Arg to Ser) mutation to remove BssH II site: 4512
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 5014-5019 & 5027-5032
pUC plasmid replication origin: 5363-6006


Notice to Purchaser:

mKate2-related materials (also referred to as "Products") are intended for research use only. The Products are covered by U.S. Pat. 7,638,615; European Pat. 1994149; and other Evrogen Patents and/or Patent applications pending. By use of these Products, you accept the terms and conditions of the applicable Limited Use Label License.

The CMV promoter is covered under U.S. Patents 5,168,062 and 5,385,839, and its use is permitted for research purposes only. Any other use of the CMV promoter requires a license from the University of Iowa Research Foundation, 214 Technology Innovation Center, Iowa City, IA 52242.

Copyright 2002-2012 Evrogen. All rights reserved.
Evrogen JSC, 16/10 Miklukho-Maklaya str., Moscow, Russia, Tel +7(495)988-4084, Fax +7(495)988-4085, e-mail:evrogen@evrogen.com