PCR cloning

PCR cloning service: terms and prices

cat.# CS031

Starting material

1. Starting materials (cDNA, RNA, genomic DNA, or plasmid DNA)

2. Vector and vector map

3. Sequence information

Service details

PROCEDURE / CAT.#		LEVEL 1 CS031-1	LEVEL 2 CS031-2
Minimal order size – € 1000. The prices do not include shipping&handling. We offer discounts for multiple orders.
1. Synthesis of primers designed for PCR amplification
2. Amplification of the target fragment
3. Cloning of fragment into the standard pUC-based vector from our collection
4a. Clone confirmation by sequencing in one direction (we will select the clone that contains the target insert, but will not verify the perfect coincidence between the sequence of the insert and the customer provided sequence information)			–
4b. Sequence verification of the perfect coincidence between the sequence of the insert and the customer provided sequence information		–
5. Plasmid purification of a correct clone from 5-10 ml culture volume
Turnaround time, weeks		agreed	agreed
Price (per fragment)	0.5-1.5 kb	€ 550	€ 1 150
	1.5-2.5 kb	€ 700	€ 1 700
	2.5-3.5 kb	€ 1 100	€ 2 500
	3.5-4.5 kb	€ 1 900	€ 3 800
	Fragment longer than 4.5 kb	Request a quote	Request a quote

Deliverables

1. PCR primers specific to the target fragment

2. Purified plasmid DNA comprising the target fragment

3. Sequencing data of the clone selected

4. Service report

5. Leftover starting material (on request)

Extra options (please specify when ordering)

OPTION	PRICE
ds cDNA preparation
Cloning of the fragment into the bacterial vector of your choice	Request a quote
Large- or mid-scale plasmid preparation	Request a quote