MOLECULAR BIOLOGY SERVICES:

    Subcloning

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Subcloning service: terms and prices

cat.# CS032

Starting material

1. Insert DNA or sequence information

2. Vector and vector map

Service details

CAT PROCEDURES TURNAROUND TIME PRICE
Minimal order size – € 1000. We offer discounts for multiple orders.
CS032 1. Isolation of the target DNA fragment (depending on your needs, we can perform (1) restriction digests to excise the target DNA fragment and isolation of the obtained fragment from low-melt agarose; (2) generating inserts by PCR; or (3) custom DNA synthesis) 4-6 weeks Request a quote
2. Modification of the target DNA fragment: construction of fusions, introduction of mutations desired, etc.
3. Ligation of the fragment into an appropriate vector
4. Transformation of the ligate from step 3 into E. coli
5. Selection of a correct clone and clone confirmation using restriction analysis or direct sequencing
6. Plasmid purification of a correct clone from 5-10 ml culture volume

Deliverables

1. Purified target insert in the selected vector

2. Service report

3. Leftover starting material (on request)

Extra options (please specify when ordering)

OPTION PRICE
Large- or mid-scale plasmid preparation Request a quote

Service pricing request on-line

 

Important Notes

Turnaround time is estimated based on average turnaround time for this type of orders. Actual time may depend on the complexity of starting materials and/or deliverables.

In all the service procedures, outcomes are highly dependent on the nature and quality of starting materials. You will be advised on any concerns we may have related to samples quality after QC tests, and every effort will be made to obtain the best possible results.

Confidentiality statement

All data and materials derived from these services are the physical and intellectual property of the purchaser and will be held strictly confidential by EVROGEN.

Copyright 2002-2011 Evrogen. All rights reserved.
Evrogen JSC, 16/10 Miklukho-Maklaya str., Moscow, Russia, Tel +7(495)988-4084, Fax +7(495)988-4085, e-mail:evrogen@evrogen.com