MOLECULAR BIOLOGY SERVICES:

    Bacterial genome subtraction

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Bacterial genome subtraction service: terms and prices

cat.# CS022

Starting material

Bacterial genomic DNA isolated from the two bacterial strains that you wish to compare

Service details

PROCEDURE / CAT.#
LEVEL 1 CS022-1
LEVEL 2 CS022-2
LEVEL 3 CS022-3
LEVEL 4 CS022-4
LEVEL 5 CS022-5
Prices include handling/shipping of service resulting materials (door-to-door delivery to one destination). Shipping of starting materials is paid by sender. We offer DISCOUNTS for MULTIPLE ORDERS.
1. Subtraction in both directions (forward = A vs B; reverse = B vs A)
2. Ligation of subtracted DNAs into an appropriate vector
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3. Transformation of the ligate from step 4 into E. coli
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4. Plating of the two subtracted libraries in 96-well plates (X plates for one direction + Y plates for the other direction)
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1 + 1
5 + 1
5 + 5
5. Differential screening of the 96-well plates with two probes to determine the percentage of differentially distributed clones in each subtracted library (probes: forward subtracted DNA, reverse subtracted DNA)
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6. Purification of plasmid DNA from the differential clones - up to 100 plasmids per each library (up to 200 plasmids in total for each SSH / 2 libraries)
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7. Southern hybridization of 5 clones from each library to confirm differential distribution
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8. Sequencing of confirmed differential clones (up to 10)
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Turnaround time, weeks
3-5
4-6
5-7
5-11
5-11
Price
€ 2 000
€ 2 800
€ 5 100
€ 6 500
€ 7 600

Deliverables

OUTPUT MATERIALS / CAT.#
LEVEL 1 CS022-1
LEVEL 2 CS022-2
LEVEL 3 CS022-3
LEVEL 4 CS022-4
LEVEL 5 CS022-5
1. Leftover starting material (on request)
2. Subtracted DNA samples and all other DNA samples generated by SSH
3. Primers for amplification of subtracted DNA and SSH products
4. Service report
5. Transformation-ready subtracted DNA ligated into an appropriate vector
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6. 96-well plates with clones used for differential screening
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7. Purified plasmid DNA from differential clones selected (1.5 μg each)
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8. Differential screening and Southern blot data
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9. Subtracted DNA libraries
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Service pricing request on-line

 

Important Notes

Turnaround time is estimated based on average turnaround time for this type of orders. Actual time may depend on the complexity of starting materials and/or deliverables.

In all the service procedures, outcomes are highly dependent on the nature and quality of starting materials. You will be advised on any concerns we may have related to samples quality after QC tests, and every effort will be made to obtain the best possible results.

Confidentiality statement

All data and materials derived from these services are the physical and intellectual property of the purchaser and will be held strictly confidential by EVROGEN.

Copyright 2002-2010 Evrogen. All rights reserved.
Evrogen JSC, 16/10 Miklukho-Maklaya str., Moscow, Russia, Tel +7(495)988-4084, Fax +7(495)988-4085, e-mail:evrogen@evrogen.com