Evrogen cDNA Depletion and Library Construction Service: Terms


Home	Research Products Product families Applications Product list How to order Technical support	Molecular Biology Services Service families Catalog items How to order	Assay Development	Licensing Opportunities Available options Contact us	Support Information How to order License statements Special offers Supplemental product information Methods of nucleic acid research Literature	About &Contacts Contact us About us Evrogen news Recent publications Citations International distributors

MOLECULAR BIOLOGY SERVICES:

cDNA depletion and library construction service: terms and prices

cat.#CS012

You provide

1. At least 1.0 μg of of total RNA or poly(A)+ RNA

See Sample requirements

2. List of the genes to be removed; up to 40 genes (sequences or GenBank IDs are required)

We perform

PROCEDURES	LEVEL 1 CAT.#CS012-1	LEVEL 2 CAT.#CS012-2
We offer DISCOUNTS for MULTIPLE ORDERS!
1. Ds cDNA preparation using SMART approach
2. cDNA depletion using duplex-specific nuclease-based method (depletion is accompanied by partial cDNA normalization)
3. PCR confirmation of the depletion
4. Ligation of the depleted cDNA into an appropriate vector ( cDNA is directionally cloned using SfiIA - SfiIB, SrfI - NotI, or AscI - NotI restriction sites)	-
5. Transformation of the ligate into E. coli and preparation of amplified depleted cDNA library; for library amplification, it is arrayed in five 25x25 cm plates (approximately 10⁵ colonies per plate); clones are eluted by LB/Amp medium; and the library is supplied with 17% glycerol and stored at –70°C	-
Turnaround time	5-7 weeks	6-8 weeks
Price	agreed	agreed

You will receive

OUTPUT MATERIALS	LEVEL 1 CAT.#CS012-1	LEVEL 2 CAT.#CS012-2

1. Any leftover starting material (on request)
2. Amplified double-stranded cDNA (at least 1 μg)
3. Depleted cDNA (at least 2 μg)
4. PCR primers for cDNA amplification
5. Amplified and characterized E.coli library	-
6. Service report

Service pricing request on-line

Important Notes

Turnaround time is estimated based on average turnaround time for this type of orders. Actual time may depend on the complexity of starting materials and/or deliverables.

In all the service procedures, outcomes are highly dependent on the nature and quality of starting materials. You will be advised on any concerns we may have related to samples quality after QC tests, and every effort will be made to obtain the best possible results.

Confidentiality statement

All data and materials derived from these services are the physical and intellectual property of the purchaser and will be held strictly confidential by EVROGEN.


Copyright 2002-2008 Evrogen. All rights reserved. Evrogen JSC, 16/10 Miklukho-Maklaya str., Moscow, Russia, +7(495)4298020, e-mail:evrogen@evrogen.com