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Normalization and library construction service: terms and pricescat.# CS011
Starting material
At least 1.0 μg of total RNA or poly(A)+ RNA
Service details (service levels may be adjusted for your particular needs)
| PROCEDURE / CAT.# |
CS011-1 LEVEL 1B |
CS011-2A LEVEL 2A |
CS011-2B LEVEL 2B |
CS011-2C LEVEL 2C |
CS011-3A LEVEL 3A |
CS011-3B LEVEL 3B |
Prices include handling/shipping of service resulting materials (door-to-door delivery to one destination). Shipping of starting materials is paid by sender. We offer DISCOUNTS for MULTIPLE ORDERS!
NOTES:
*Library cloning is performed into a vector from our collection using SfiIA - SfiIB, SrfI - NotI, or AscI - NotI restriction sites. Cloning using other sites or into a customer-provided vector can be performed at the agreed price.
**To prepare an amplified cDNA library, the non-amplified library is arrayed in five 25x25 cm plates (approximately 105 colonies per plate); clones are eluted by LB/Amp medium; then the library is supplied with 17% glycerol and stored at -70°C. Preparation of the normalized amplified cDNA library containing more than 400 000 independent clones can be performed at the agreed price.
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| 1. Synthesis of ds cDNA suitable for directional cloning |
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| 2. DSN-normalization of cDNA |
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| 3. Ligation of normalized cDNA into an appropriate vector* |
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| 4. PCR quality control of 22 randomly picked clones |
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| 5a. Preparation of non-amplified normalized cDNA library containing at least 100 000 independent clones |
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| 5b. Preparation of amplified normalized cDNA library containing at least 400 000 independent clones** |
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| 6. Purification of plasmid DNA from the 90 clones |
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| 7. Single run sequencing analysis of these clones |
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| Turnaround time, weeks |
4-6 |
4-6 |
5-7 |
5-7 |
6-8 |
6-8 |
| Price |
€ 2 550 |
€ 3 700 |
€ 4 500 |
€ 4 500 |
€ 5 900 |
€ 5 900 |
Extra options
| OPTION |
DESCRIPTION |
PRICE |
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| Normalization efficiency analysis |
Virtual Northern blot is performed for samples from human and mouse with ACTB and UBC genes-derived probes (high abundance in most tissues). For samples from other sources, PCR analysis is used, if sequences of two abundant transcripts are provided by a customer; or Virtual Northern blot is used, if two cloned fragments corresponding to abundant transcripts are provided |
agreed |
| Preparation of additional normalized cDNA |
up to 5 μg |
€ 350 |
| up to 10 μg |
€ 800 |
| up to 15 μg |
€ 1 250 |
Deliverables
| OUTPUT MATERIALS / CAT.# |
CS011-1 LEVEL 1B |
CS011-2A LEVEL 2A |
CS011-2B LEVEL 2B |
CS011-2C LEVEL 2C |
CS011-3A LEVEL 3A |
CS011-3B LEVEL 3B |
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| 1. Amplified non-normalized cDNA (at least 1 μg) |
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| 2. Normalized cDNA (at least 2 μg) |
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| 3. PCR primers for cDNA amplification |
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| 4. Service report |
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| 5. Normalized cDNA ligated into a vector |
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| 6. Non-amplified normalized cDNA library (at least 100 000 independent clones) |
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| 7. Amplified normalized cDNA library (at least 400 000 independent clones) |
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| 8. Purified plasmid DNA from the 90 clones and insert sequencing data of these 90 clones |
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Important Notes
Turnaround time is estimated based on average turnaround time for this type of orders. Actual time may depend on the complexity of starting materials and/or deliverables.
In all the service procedures, outcomes are highly dependent on the nature and quality of starting materials. You will be advised on any concerns we may have related to samples quality after QC tests, and every effort will be made to obtain the best possible results.
Confidentiality statement
All data and materials derived from these services are the physical and intellectual property of the purchaser and will be held strictly confidential by EVROGEN.
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