MOLECULAR BIOLOGY SERVICES:

cDNA subtraction and library construction service: terms and prices

cat.#CS021

You provide

At least 1.0 μg of of total RNA or poly(A)+ RNA

See Sample requirements

We perform

PROCEDURES / CAT.#
LEVEL 1

CS021-1
LEVEL 2

CS021-2
LEVEL 3

CS021-3
LEVEL 4

CS021-4
LEVEL 5

CS021-5
The prices include shipping&handling. We offer DISCOUNTS for MULTIPLE ORDERS!
1. ds cDNA preparation
2. Subtractive hybridization in both directions
2'. Mirror orientation selection (optional, on request)
3. Ligation of subtracted cDNAs into an appropriate vector
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4. Transformation of the ligate from step 3 into E. coli
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5. Plating of the two subtracted libraries in 96-well plates (X plates for the one direction + Y plates for the second direction)
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1 + 1
5 + 1
5 + 5
6. Differential screening of the 96-well plates with two probes to determine the percentage of differentially expressed clones in each subtracted library (probes: forward subtracted cDNA, reverse subtracted cDNA)
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7. Purification of plasmid DNA from the differential clones - up to 100 plasmids per each library (up to 200 plasmids in total for each SSH / 2 libraries)
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8. Virtual Northern hybridization of 5 clones from each library to confirm differential expression
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9. Sequence of confirmed differential clones (up to 10)
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Turnaround time, weeks
3-5
4-6
5-7
5-11
5-11
Price
€ 1 700
€ 2 500
€ 5 100
€ 6 500
€ 7 600
MOS adds € 1 000 to the order price

You will receive

PROCEDURE / CAT.#
LEVEL 1

CS021-1
LEVEL 2

CS021-2
LEVEL 3

CS021-3
LEVEL 4

CS021-4
LEVEL 5

CS021-5
1. Any leftover starting materials (on request)
2. Amplified tester and driver cDNAs (at least 0.5 μg)
3. Subtracted cDNA samples and all other cDNA samples generated by SSH (at least 1 μg)
4. Primers for amplification of subtracted cDNA and SSH products
5. Service report
6. Transformation-ready subtracted cDNA ligated into an appropriate vector
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7. 96-well plates with clones used for differential screening
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8. Purified plasmid DNA from the differential clones (at least 1.5 μg each)
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9. Differential screening and Virtual Northern blot data
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10. Subtracted cDNA libraries with descriptions (number of clones and percentage of clones with insertions)
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Service pricing request on-line

 

Important Notes

Turnaround time is estimated based on average turnaround time for this type of orders. Actual time may depend on the complexity of starting materials and/or deliverables.

In all the service procedures, outcomes are highly dependent on the nature and quality of starting materials. You will be advised on any concerns we may have related to samples quality after QC tests, and every effort will be made to obtain the best possible results.

Confidentiality statement

All data and materials derived from these services are the physical and intellectual property of the purchaser and will be held strictly confidential by EVROGEN.

Copyright 2002-2008 Evrogen. All rights reserved.
Evrogen JSC, 16/10 Miklukho-Maklaya str., Moscow, Russia, +7(495)4298020, e-mail:evrogen@evrogen.com