Whole-genome sequencing (WGS) is a powerful approach for obtaining reference sequence information for multiple organisms. In the case of large genomes, presence of repetitive sequences, which constitute a considerable proportion of total genome size, makes WGS impractical. Elimination of the repetitive DNAs facilitates sequencing and analysis of large genomes.

Evrogen duplex-specific nuclease (DSN)-based normalization technology is a highly efficient and well proved approach to equalize transcript abundance in cDNA populations enriched with full-length sequences. DSN-normalization applied for genomic DNA is effective against abundant repetitive elements with high sequence identity and satellite DNAs, while retains highly divergent repeats and coding regions at baseline levels (Shagina et al., 2010). In other words, DSN-normalization can be used to eliminate evolutionarily young repetitive sequences from genomic DNA before sequencing, and should prove invaluable in studies of large eukaryotic genomes, such as those of higher plants.