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RACE and full-length cDNA isolation service: terms and prices

cat.# CS033

Starting material

1. At least 1.0 μg of total RNA or poly(A)+ RNA

2. Sequence information

Service details

CAT PROCEDURES TURNAROUND TIME PRICE
Minimal order size – € 1000. We offer discounts for multiple orders.
CS033 1. ds cDNA preparation using SMART approach 4 weeks or longer depending on project complexity Request a quote
2. Construction of oligonucleotide primers (gene-specific or degenerative) on the customer-supplied sequence of interest
3. Amplification of the target cDNA ends (3'- and 5'- RACE)
4. Cloning of the target cDNA ends into an appropriate vector
5. Partial sequencing of the cloned cDNA fragments
6. Construction of oligonucleotide primers to amplify the target full-length cDNA
7. Amplification of the target full-length cDNA
8. Cloning of the target full-length cDNA into an appropriate vector and screening of the clones to select those containing target full-length cDNA inserts
9. Purification of vector DNA with the target full-length cDNA insert

Deliverables

1. First strand cDNA (with specific adapters on both ends, on request)

2. Amplified double-stranded cDNA (1 μg)

3. PCR primers for cDNA amplification

4. PCR products generated by RACE (0.5 μg each, on request)

5. Purified vector containing the target full-length cDNA insert

6. All primers designed and synthesized during RACE

7. Service report

8. Leftover starting material (on request)

Extra options (please specify when ordering)

OPTION PRICE
Complete sequencing of the target DNA Request a quote

Service pricing request on-line

 

Important Notes

Turnaround time is estimated based on average turnaround time for this type of orders. Actual time may depend on the complexity of starting materials and/or deliverables.

In all the service procedures, outcomes are highly dependent on the nature and quality of starting materials. You will be advised on any concerns we may have related to samples quality after QC tests, and every effort will be made to obtain the best possible results.

Confidentiality statement

All data and materials derived from these services are the physical and intellectual property of the purchaser and will be held strictly confidential by EVROGEN.

Copyright 2002-2011 Evrogen. All rights reserved.
Evrogen JSC, 16/10 Miklukho-Maklaya str., Moscow, Russia, Tel +7(495)988-4084, Fax +7(495)988-4085, e-mail:evrogen@evrogen.com