	Normalization significantly increases gene discovery rate of cDNA library. Transcript distribution in standard and normalized cDNA libraries from Aplysia neurons: blue – unique sequences; green – non-unique sequences, yellow – all sequences.

Normalization significantly increases gene discovery rate of cDNA library.

Transcript distribution in standard and normalized cDNA libraries from Aplysia neurons: blue – unique sequences; green – non-unique sequences, yellow – all sequences.


Typical cDNA normalization result. (A) Agarose/EtBr gel-electrophoresis of non-normalized (1) and normalized (2) human cDNA samples; (B, C) concentration of abundant transcripts in these samples revealed by Virtual Northern blot. ACTB – β-actin; UBC – ubiquitin C; M, 1-kb DNA size markers (SibEnzyme); embr. – embryonic.

Typical cDNA normalization result.

(A) Agarose/EtBr gel-electrophoresis of non-normalized (1) and normalized (2) human cDNA samples; (B, C) concentration of abundant transcripts in these samples revealed by Virtual Northern blot.

ACTB – β-actin; UBC – ubiquitin C; M, 1-kb DNA size markers (SibEnzyme); embr. – embryonic.