
Typical cDNA subtraction and differential screening results. (A) Agarose/EtBr gel electrophoresis of unsubtracted (1,2); subtracted with SHH (#1 – 1 vs 2; #2 – 2 vs 1) cDNA samples and the samples after MOS procedure; (B, C) Differential screening results of randomly picked clones from #1' sample with radiolabeled subtracted cDNA probes: (B) #1'-probe; (C) #2'-probe.

Typical cDNA subtraction and differential screening results.

(A) Agarose/EtBr gel electrophoresis of unsubtracted (1,2); subtracted with SHH (#1 – 1 vs 2; #2 – 2 vs 1) cDNA samples and the samples after MOS procedure; (B, C) Differential screening results of randomly picked clones from #1' sample with radiolabeled subtracted cDNA probes: (B) #1'-probe; (C) #2'-probe.