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TagRFP
SUPPORTRESOURCES |
Red fluorescent protein TagRFP- Bright red (orange) fluorescence Performance and useTagRFP can be easily expressed and detected in a wide range of organisms. Mammalian cells transiently transfected with TagRFP expression vectors produce bright fluorescence in 10-12 hrs after transfection. No cytotoxic effects or visible protein aggregation are observed. High pH-stability with pKa=3.8 makes it possible to use TagRFP for imaging in acidic organelles, such as late and recycling endosomes and lysosomes.
TagRFP performance in protein fusions has been demonstrated in fibrillarin, vinculin, zyxin, TagRFP suitability to generate stably transfected cells has been proven by Marinpharm company. TagRFP can be used in multicolor labeling applications with blue, cyan, green, yellow, and far-red fluorescent dyes. TagRFP is ideally suitable for use as an acceptor for FRET with Evrogen green fluorescent protein TagGFP2. The calculated Forster distance (R0 = 5.7 nm) for the TagGFP2-TagRFP pair is one of the largest among the values reported. At the same time, since TagGFP2 and TagRFP emission peaks are spaced by as much as 78 nm, the emission signal for these two proteins can be easily separated in any imaging system. High pH-stability of the both proteins allows using this pair for imaging in acidic organelles.
The excellent performance of TagRFP in FRET application was demonstrated both in vitro and in vivo on the example of FRET-based apoptosis reporter References:
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