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The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced. |
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Location of features
PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Histone H2B: 657-1034
TagRFP: 1053-1766
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1919-1924 & 1948-1953
mRNA 3' ends: 1957 & 1969
f1 single-strand DNA origin: 2016-2471
Bacterial promoter for expression of Kanr gene
-35 region: 2533-2538
-10 region: 2556-2561
Transcription start point: 2568
SV40 origin of replication: 2812-2947
SV40 early promoter
Enhancer (72-bp tandem repeats): 2645-2716 & 2717-2788
21-bp repeats: 2792-2812, 2813-2833 & 2835-2855
Early promoter element: 2868-2874
Major transcription start points: 2864, 2902, 2908 & 2913
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2996-2998
Stop codon: 3788-3790
G->A mutation to remove Pst I site: 3178
C->A (Arg to Ser) mutation to remove BssH II site: 3524
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4026-4031 & 4039-4044
pUC plasmid replication origin: 4375-5018
TagRFP-related materials (also referred to as "Products") are intended for research use only. The Products are covered by U.S. Pat. 7,638,615; European Pat. 1994149; and other Evrogen Patents and/or Patent applications pending. By use of these Products, you accept the terms and conditions of the applicable Limited Use Label License.
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