| Variant | Description | Related vector | Cat.# | Click for image |
|---|---|---|---|---|
| ||||
| PhiYFP | PhiYFP codon usage is optimized for high expression in mammalian cells [Haas et al., 1996], but it can be successfully expressed in many other heterological systems. PhiYFP allows generation of fusions to its N-terminus but unsuited to generate C-terminal fusions. |
|
FP602 |
|
|
|
FP604 | |||
| PhiYFP-mito fusion | A mitochondrial targeting sequence (MTS) is fused to the PhiYFP N-terminus. MTS was derived from the subunit VIII of human cytochrome C oxidase [Rizzuto et al., 1989; Rizzuto et al., 1995]. When expressed in mammalian cells, this variant provides green fluorescent labeling of mitochondria. |
|
FP607 |
|
| PhiYFP-m-peroxi fusion | Peroxisomal targeting signal [Gould et al., 1989] encoding tripeptide SKL was fused to the 3' end of PhiYFP-m sequence. This tripeptide targets the fusion protein to the matrix of peroxisomes. |
|
FP606 |
|