Available variants and fusions

Variant	Description	Related vector	Cat.#	Click for image
Humanized TagRFP	TagRFP codon usage is optimized for high expression in mammalian cells [Haas et al., 1996], but it can be successfully expressed in many other heterological systems.	pTagRFP-C	FP141
		pTagRFP-N	FP142
TagRFP-AS variant	TagRFP-AS codon usage is optimized for high expression in Arabidopsis and Saccharomyces. This variant is available in Gateway® entry clones.	Gateway® TagRFP-AS-C entry clone	FP148
		Gateway® TagRFP-AS-N entry clone	FP149
TagRFP-tubulin fusion	Human α-tubulin is fused to the TagRFP C-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of α-tubulin in living cells.	pTagRFP-tubulin	FP145
TagRFP-mito fusion	A mitochondrial targeting sequence (MTS) is fused to the TagRFP N-terminus. MTS was derived from the subunit VIII of human cytochrome C oxidase [Rizzuto et al., 1989; Rizzuto et al., 1995]. When expressed in mammalian cells, this variant provides red (orange) fluorescent labeling of mitochondria.	pTagRFP-mito	FP147
TagRFP-Cx43 fusion	Rat connexin 43 is fused to the TagRFP N-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of connexin 43 in living cells.	pTagRFP-Cx43	FP364
TagRFP-Golgi fusion	Golgi targeting sequence (fragment of human β-1,4-galactosyltransferase) is fused to the TagRFP N-terminus. When expressed in mammalian cells, this variant provides red (orange) fluorescent labeling of Golgi apparatus.	pTagRFP-Golgi	FP367
TagRFP-H2B fusion	Human histone H2B is fused to the TagRFP N-terminus. When expressed in mammalian cells, this fusion provides red (orange) fluorescent labeling of histone H2B in living cells.	pTagRFP-H2B	FP368