Ranging in color from blue to far-red, Evrogen fluorescent proteins can be used in fluorescence resonance energy transfer (FRET) applications as donors and acceptors of fluorescence. TagFPs are perfect for in vivo protein interaction studies by FRET due to their improved performance in fusions.
TagBFP-TagGFP2 and TagGFP2-TagRFP pairs show the highest FRET efficiency among the tested TagFPs combinations and compare favorably to other available FRET pairs of monomeric fluorescent proteins.