RESEARCH PRODUCTS/Basic fluorescent proteins:

TagFPs: protein localization tags

- Bright monomeric proteins; excellent performance in fusions
- Wide color palette
- For research and commercial use

TagFPs group comprises proteins that are optimized for protein localization / interaction studies and stable expression in long term cultures. They can be also used for other cell labeling applications.
TagFPs demonstrate successful performance in fusions with cellular proteins and can be expressed in various heterological systems. Ranging in color from cyan to far-red, they provide unique possibilities for multicolor labeling of subcellular structures.

ready-to-use vectors for protein labeling

 

Spectral diversity

TagFPs excitation spectra. TagFPs emission spectra.

Normalized excitation / emission spectra.

Enlarge
Available proteins
CHARACTERISTIC TagCFP TagGFP TagYFP TagRFP TagFP635
* Brightness is a product of extinction coefficient and quantum yield, divided by 1000
Fluorescence colorcyangreenyellowred (orange)far-red
Excitation max (nm)458482508555588
Emission max (nm)480505524584635
Quantum yield0.570.590.620.480.33
Extinction coeff. (M-1cm-1)37 00058 20064 000100 00045 000
Brightness*21.134.339.748.014.9
Brightness, % of EGFP64 %104 %120 %145 %45%
pKa4,74.75.53.86.0
Structuremonomermonomermonomermonomermonomer
Cell toxicitynot observednot observednot observednot observednot observed
Aggregationnonononono
Maturation rate at 37°Cfastfastfastfastfast
Molecular weight (kDa)27 27 27 27 27
Copyright 2002-2008 Evrogen. All rights reserved.
Evrogen JSC, 16/10 Miklukho-Maklaya str., Moscow, Russia, +7(495)4298020, e-mail:evrogen@evrogen.com