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TagFPs: protein localization tags

- Bright monomeric proteins; excellent performance in fusions
- Wide color palette
- For research and commercial use

TagFPs group comprises proteins that are optimized for protein localization / interaction studies and stable expression in long term cultures. They can be also used for other cell labeling applications.
TagFPs demonstrate successful performance in fusions with cellular proteins and can be expressed in various heterological systems. Ranging in color from blue to far-red, they provide unique possibilities for multicolor labeling of subcellular structures.

FRET pairs

 

ready-to-use vectors for protein labeling

 

Spectral diversity

TagFPs excitation spectra. TagFPs emission spectra.

Normalized excitation / emission spectra

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Available proteins
CHARACTERISTIC TagBFPTagCFPTagGFP2TagYFPTagRFPmKate2
* Brightness is a product of extinction coefficient and quantum yield, divided by 1000.
Ext.coeff. – extinction coefficient.
Fluorescence colorblue cyan green yellow red (orange) far-red
Excitation max (nm)402 458 483 508 555 588
Emission max (nm)457 480 506 524 584 633
Quantum yield0.63 0.57 0.6 0.62 0.48 0.40
Ext. coeff. (M-1cm-1)52 000 37 000 56 500 50 000 100 000 62 500
Brightness*32.8 21.1 33.9 31 48.0 25.0
Brightness, % of EGFP99 64 105 94 148 74
pKa2.7 4.7 5.0 5.5 3.8 5.4
Structuremonomer monomer monomer monomer monomer monomer
Cell toxicitynot observed not observed not observed not observed not observed not observed
Aggregationno no no no no no
Maturation rate at 37°Cfast fast fast fast fast fast
Molecular weight (kDa)26 27 27 27 27 26
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