Tools for labeling of subcellular structures

- Easy labeling of cell compartments and proteins
- Wide collection of fluorescent colors
- No exogenous chemical compounds or cofactors required

Ready-to-use expression vectors are available for labeling

Organelles	Cytoskeletal and adhesion proteins	Gap junction proteins	Nucleolar proteins
Golgi apparatus  mitochondria  peroxisomes	α-actinin   α-V-Integrin  α-tubulin  β-actin EB3 protein  focal adhesion kinase  cytokeratin-18  profilin  vinculin  zyxin	connexin 26  connexin 32  connexin 43  Vesicular transport proteins clathrin light chain LCB	histone H2B  lamin B1

Evrogen collection of fluorescent proteins provides wide opportunities for subcellular imaging. In addition to ready-to-use subcellular localization vectors, vectors comprising fluorescent protein coding sequence linked with multiple cloning sites allow easy targeting of the reporter in the specific cellular compartment. The targeting signal can be fused to C- or N- end of the fluorescent protein using appropriate expression vector (marked with "-C" or "-N" correspondingly). Unmodified vectors can be used for cytosolic expression of fluorescent markers.

Fluorescent microscopy of mammalian cells expressing Evrogen fluorescent proteins targeted to the different subcellular structures and proteins