Molecular biology services
Transcriptome research

Whole transcriptome sequencing, RNA-seq New!

Preparation of uncloned cDNA for next generation sequencing (from prokaryotic and eukaryotic RNA)

Double-stranded amplified cDNA

Normalized cDNA

Subtracted cDNA

Ribosomal cDNA depletion

Custom cDNA libraries

Full-length-enriched cDNA libraries

Normalized full-length-enriched cDNA libraries

Depleted full-length-enriched cDNA libraries

Subtracted cDNA libraries

Genome research

Whole genome sequencing New!

Genomic DNA normalization for next generation sequencing

Bacterial genome subtraction

Eukaryotic genome subtraction

Immunome research New!

T-Cell Receptor (TCR) profiling

Routine laboratory services

Amplification and cloning

PCR cloning

Subcloning

Gene synthesis and modification

Gene synthesis

Site-directed mutagenesis

Custom optimization of Evrogen expression vectors

Services in focus

Next-generation sequencing
A complete pipeline for analysis of your RNA or DNA samples including a wide variety of transcriptome and genome research techniques in combination with next-generation sequencing and bioinformatics.

Ribosomal cDNA depletion
Non-mRNA-derived cDNAs are depleted while rare mRNA-derived cDNAs are unaffected. Output cDNA samples are suitable for next generation sequencing. The method is optimized for both eukaryotic and prokaryotic total RNA.

T-Cell Receptor (TCR) profiling
Comprehensive study of T-Cell Receptor (TCR) diversity in human tissues (e.g. blood) to estimate an immune system status and its changes under conditions of autoimmune disease or infection.

Genomic DNA normalization
Effective elimination of high-abundant young repetitive elements and satellite DNA from genomic DNA.
Normalized DNA is suitable for further next generation sequencing.

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Evrogen JSC, 16/10 Miklukho-Maklaya str., Moscow, Russia, Tel +7(495)988-4084, Fax +7(495)988-4085, e-mail:evrogen@evrogen.com