|
Antibleaching live cell visualization medium DMEMgfp
- Recommended for visualization of cells expressing green fluorescent proteins
 |
Photobleaching of fluorescent proteins in response to prolonged exposure to exciting radiation significantly affects their utility as in vivo labels. The only ways of minimizing photobleaching known to date were limited to reducing of the excitation light intensity or shortening the duration of illumination. New Evrogen DMEMgfp live cell visualization medium excludes the components that lead to oxidative redding of green fluorescent proteins, the photochemical process that was shown to be one of the main sources of GFP photobleaching [Bogdanov et al., 2009]. Replacing the culture medium with DMEMgfp for the period of visualization results in up to a 9-fold increase of photostability of EGFP, a 3.3-fold increase of photostability of TagGFP2 and more than a 4-fold increase of photostability of activated forms of photoactivatable PA-GFP and PS-CFP2 (the effect varies for particular fusions and localizations).
|
DMEMgfp medium is intended for imaging use only, and is not recommended for long-term cell culturing. Nevertheless, no negative effects on cell physiology were observed during the first week of cultivation in DMEMgfp.
Use
Replace your cell culture medium with DMEMgfp 20-30 min before starting imaging experiment. The replacement should be performed under sterile conditions.
 |  | Long-term fluorescent microscopy of EGFP in live HEK293T cells maintained in DMEM or DMEMgfp.
|
|---|
 |  |  | |
|---|
Influence of cell medium on photostability of fluorescent proteins.
(A) - EGFP, (B) AcGFP1, (C) TagGFP2. Graph shows normalized bleaching curves of fluorescent proteins in live HEK293 cells maintained in DMEM (black squares), or DMEMgfp (green triangles). Standard deviations (n = 15-20 cells) are shown.
|
 |  | HeLa cells transfected with fluorescent protein-tagged α-tubulin or β-actin had a normal cytoskeleton after 5-day culture in DMEMgfp.
Fluorescence microscopy of TagGFP2-α-tubulin and mKate2-β-actin of live HeLa cells after 5 day culturing in DMEMgfp.
|
|---|
Available products| PRODUCT | CAT.# | DESCRIPTION | SIZE | PRICE | CERTIFICATE OF FORMULATION (PDF) |
|---|
| The price does not include delivery. Currently available via Axxora LLC only. |
| DMEMgfp |
MC101 |
antibleaching live cell visualization medium |
100ml |
€ 55 |
 |
Storage: Should be stored at +2 - 8°C
Notice to Purchaser:
Evrogen Cell Medium Products (the Products) are intended for research use only. The Products are covered by Evrogen Patents and/or Patent applications pending.
MATERIAL SAFETY DATA SHEET INFORMATION
To the best of our knowledge, these products do not require a Material Safety Data Sheet. However, all the properties of these products (and, if applicable, each of their components) have not been thoroughly investigated. Therefore, we recommend that you use gloves and eye protection, and wear a laboratory coat when working with these products.
References:
-
Bogdanov AM, Bogdanova EA, Chudakov DM, Gorodnicheva TV, Lukyanov S, Lukyanov KA.
Cell culture medium affects GFP photostability: a solution.
Nat Methods. 2009; 6 (12):859-60. / pmid: 19935837
|
