Antibleaching live cell visualization medium DMEM^gfp

- Recommended for visualization of cells expressing green fluorescent proteins

Photobleaching of fluorescent proteins in response to prolonged exposure to exciting radiation significantly affects their utility as in vivo labels. The only ways of minimizing photobleaching known to date were limited to reducing of the excitation light intensity or shortening the duration of illumination. New Evrogen DMEMgfp live cell visualization medium (referred to as DMEM-V in Bogdanov et al., 2009) excludes the components that lead to oxidative reddening of green fluorescent proteins, the photochemical process that was shown to be one of the main sources of GFP photobleaching.

Replacing the culture medium with DMEM^gfp for the period of visualization results in up to a 9-fold increase of photostability of EGFP, a 3.3-fold increase of photostability of TagGFP2 and more than a 4-fold increase of photostability of activated forms of photoactivatable PA-GFP and PS-CFP2 (the effect varies for particular fusions and localizations).

DMEM^gfp medium is intended for imaging use only, and is not recommended for long-term cell culturing. Nevertheless, no negative effects on cell physiology were observed during the first week of cultivation in DMEM^gfp supplemented with L-glutamine, penicillin, streptomycin and fetal bovine serum. These common supplementary compounds have no influence on antibleaching properties of the medium.

Use

Replace your cell culture medium with DMEM^gfp 20-30 min before starting imaging experiment. The replacement should be performed under sterile conditions. If necessary, DMEM^gfp can be supplemented with L-glutamine, penicillin, streptomycin and fetal bovine serum.

	Long-term fluorescent microscopy of EGFP in live HEK293T cells maintained in DMEM or DMEMgfp.

Influence of cell medium on photostability of fluorescent proteins. (A) – EGFP, (B) AcGFP1, (C) TagGFP2. Graph shows normalized bleaching curves of fluorescent proteins in live HEK293 cells maintained in DMEM (black lines), or DMEMgfp (green lines). Standard deviations (n = 15-20 cells) are shown.

		HeLa cells transfected with fluorescent protein-tagged α-tubulin or β-actin had a normal cytoskeleton after 5-day culture in DMEMgfp. Fluorescence microscopy of TagGFP2-α-tubulin and mKate2-β-actin of live HeLa cells after 5 day culturing in DMEMgfp.

Available products

PRODUCT	CAT.#	DESCRIPTION	SIZE	PRICE	CERTIFICATES OF FORMULATION
The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information.
DMEMgfp	MC101	antibleaching live cell visualization medium	100ml	€ 55

Storage: Should be stored at +4 – +8°C. DO NOT FREEZE.