Services in focus

Ribosomal cDNA depletion
Non-mRNA-derived cDNAs are depleted while rare mRNA-derived cDNAs are unaffected. Output cDNA samples are suitable for next generation sequencing. The method is optimized for both eukaryotic and prokaryotic total RNA.

T-Cell Receptor (TCR) profiling
Comprehensive study of T-Cell Receptor (TCR) diversity in human tissues (e.g. blood) to estimate an immune system status and its changes under conditions of autoimmune disease or infection.

Genomic DNA normalization
Effective elimination of high-abundant young repetitive elements and satellite DNA from genomic DNA.
Normalized DNA is suitable for further next generation sequencing.

Preparation of normalized cDNA for further next generation sequencing
New options tailored specifically for sequencing using Roche 454, Illumina Solexa, ABI SOLiD technologies.

Molecular biology services
Numerical index

CAT.#

SERVICE

CS010

Preparation of uncloned normalized cDNA for next generation sequencing

CS011

Construction of normalized cDNA libraries

CS012

cDNA depletion and library construction

CS013

Genomic DNA normalization

CS014

Ribosomal cDNA depletion

CS021

Eukaryotic cDNA subtraction and library construction service

CS022

Bacterial genome subtraction service

CS023

Eukaryotic genome subtraction service

CS024

Prokaryotic cDNA subtraction and library construction service

CS030

cDNA synthesis and amplification for next generation sequencing

CS031

PCR and RT-PCRcloning service

CS032

Subcloning service

CS033

RACE and isolation of full-lenght cDNA service

CS035

Genome walking service

CS040

Standard cDNA library construction service

CS041

Site-directed mutagenesis service

CS042

Gene synthesis service

CS061

T-Cell Receptor (TCR) profiling: cDNA preparation

CS062

T-Cell Receptor (TCR) profiling: bioinformatics analysis

FPS00

Custom optimization of Evrogen expression vectors

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Evrogen JSC, 16/10 Miklukho-Maklaya str., Moscow, Russia, Tel +7(495)988-4084, Fax +7(495)988-4085, e-mail:evrogen@evrogen.com