RESEARCH PRODUCTS/ Nucleic Acid Research:

Mint and Mint-Universal cDNA synthesis kits

cat.# SK001; SK002

- Fast cDNA synthesis protocol
- High content of full length transcripts
- Low background
- Free trial-size Encyclo PCR kit included

Mint and Mint-Universal cDNA synthesis kits are designed to synthesize full-length-enriched double stranded (ds) cDNA from total or poly(A)+ RNA.
cDNA prepared by these kits are flanked by known adapter sequences at the both ends and can be used for various applications including construction of cDNA libraries, virtual Northern blot, subtractive hybridization, array probe preparation.

technology overview

 

Mint kit allows synthesis of cDNA flanked by the same adapter sequences at the both 3'- and 5'- ends. This cDNA is suitable for preparation of non-directionally cloned cDNA libraries; virtual Northern blot (Frantz et al., 1999); supression subtractive hybridization (SSH, Diatchenko et al., 1996). The cDNA can be normalized before cloning using Trimmer kit (Cat.#NK001).

Mint-Universal kit comprises reagents and protocols for synthesis of cDNA flanked by the same (protocol 1) or different (protocol 2) adapter sequences. In addition to all applications noted above, this cDNA can be used for generation of directionally cloned cDNA libraries. Depending on the synthesis protocol, Mint-Universal generated cDNA can be normalized using either Trimmer kit (Cat.# NK001) or Trimmer-direct kit (Cat.# NK002) before library cloning.

Mint-amplified cDNA from different sources.

1 - mouse liver; 2 - mouse skeletal muscle; 3 - mouse brain; 4 - human leucocytes; 5 - human lung; 6 - human skeletal muscle; 7 - mosquito grub; 8 - copepod Pontella sp.; 9 - tomato Lycopersicon esculentum. M - 1 kb DNA size marker, SibEnzyme, Russia.

Available products

ProductCat.# Description SizePrice User Manual (pdf)
Mint kit SK001 Each package comprises a detailed instruction, reagents for cDNA synthesis, free trial-size Encyclo PCR kit, and a control RNA sample 20 rxn € 600.00
Mint-Universal kit SK002 Each package comprises a detailed instruction, reagents for cDNA synthesis, free trial-size Encyclo PCR kit, and a control RNA sample 20 rxn € 700.00
The prices do not include delivery. The prices vary in different countries. Mint and Mint-Universal kits are not available in certain countries. Please contact your local distributor before ordering.

Storage: Should be stored at -20°C

References:

  • Diachenko L, Lau YF, Campbell AP, Chenchik A, Mogadam F, Huang B, Lukyanov S, Lukyanov K, Gurskaya N, Sverdlov ED, Siebert D. (1996) Suppression Subtracive Hybridization: A method for generating differentially regulated or tissue-specific cDNA probes and libraries. Proc. Natl. Acad. Sci. USA 93(12): 6025-6030. / PMID:8650213
  • Franz, O., Bruchhaus, I.I, Roeder, T. (1999) Verification of differential gene transcription using virtual northern blotting. Nucleic Acids Res. 27: e3.

Notice to Purchaser:

Evrogen nucleic acid-related products are intended for research use only. The products are covered by Evrogen Patents and/or Patent applications pending. By use of these products, you accept the terms and conditions of the applicable Limited Use Label License #002.

PCR is the subject of patents issued in certain countries. The purchase of this kit does not include a license to perform PCR. However, many researchers may not be required to obtain a license. Other investigators may already have a license to perform PCR through use of a thermal cycler with the appropriate label license.

MATERIAL SAFETY DATA SHEET INFORMATION

To the best of our knowledge, these products do not require a Material Safety Data Sheet. However, all the properties of these products (and, if applicable, each of their components) have not been thoroughly investigated. Therefore, we recommend that you use gloves and eye protection, and wear a laboratory coat when working with these products.

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