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    Katushka2S

Far-red fluorescent protein Katushka2S

- Super bright far-red fluorescence
- High signal-to-noise ratio, fast maturation
- Recommended for whole body imaging

Katushka2S is the next generation of far-red fluorescent protein TurboFP635 (Katushka) [Luker et al., 2015; Shcherbo et al., 2009; Gurskaya et al., 2011]. Katushka2S exhibits highest brightness, fastest maturation and superior signal-to-noise ratio compared to TurboFP635 and many other available far-red fluorescent proteins: E2-Crimson, mNeptune, mNeptune2.5, mCardinal, TurboFP650 and NirFP [Luker et al., 2015], which makes it the protein of choice for visualization within living tissues. Katushka2S can also be used together with iRFP720 [Luker et al., 2015] for dual color whole body imaging.

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*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
The prices do not include delivery. The prices vary in different countries. Please contact your local distributor for exact prices and delivery information.


Katushka2S expression/source vectors

pKatushka2S-C FP761 Mammalian expression vector encoding humanized Katushka2S and allowing its expression and generation of fusions to the Katushka2S C-terminus 20 μg € 480 / 240*
pKatushka2S-B FP763 Bacterial expression vector; source of the Katushka2S coding sequence 20 μg € 480 / 240*


Antibody against Katushka2S

Anti-tRFP
antibody
AB233 Rabbit polyclonal antibody against TurboRFP, TurboFP602, TurboFP635, Katushka2S, TurboFP650, NirFP, TagBFP, TagRFP, FusionRed, TagFP635, mKate2, PA-TagRFP, mRuby and mCherry 100 μg € 220

References:

  • Gurskaya NG, Staroverov DB, Fradkov AF, Lukyanov KA. Coding region of far-red fluorescent protein katushka contains a strong donor splice site. Bioorg Khim. 2011; 37 (3):425-8. (in Russian) / pmid: 21899059
  • Luker KE, Pata P, Shemiakina II, Pereverzeva A, Stacer AC, Shcherbo DS, Pletnev VZ, Skolnaja M, Lukyanov KA, Luker GD, Pata I, Chudakov DM. Comparative study reveals better far-red fluorescent protein for whole body imaging. Sci Rep. 2015; 5 :10332. doi: 10.1038/srep10332 / pmid: 26035795
  • Shcherbo D, Murphy CS, Ermakova GV, Solovieva EA, Chepurnykh TV, Shcheglov AS, Verkhusha VV, Pletnev VZ, Hazelwood KL, Roche PM, Lukyanov S, Zaraisky AG, Davidson MW, Chudakov DM. Far-red fluorescent tags for protein imaging in living tissues. Biochem J. 2009; 418 (3):567-74. doi: 10.1042/BJ20081949 / pmid: 19143658
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