Licensing opportunities: basic fluorescent proteins

- Multicolor protein collection for in vivo labeling applications
- Direct expression in cells
- For research and commercial use

Evrogen offers proprietary collection of fluorescent proteins optimized for various applications in commercial biotechnology, cell biology and biomedicine.

Diversity of Evrogen basic fluorescent proteins

Ranging in color from cyan to far-red, Evrogen fluorescent proteins can be used for multicolor labeling to observe different cellular events in a particular cell or a cell population.

TurboFPs excitation spectra. TurboFPs emission spectra.

Spectral diversity

Normalized excitation/emission spectra.
Enlarge

List of fluorescent proteins
PRODUCT PAGE COLOR EX/EM MAX (nm) BRIGHTNESS,
% OF EGFP
STRUCTURE MATURATION RATE AT 37°C DESCRIPTION (PDF)

TagFPs, protein localization tags / details
TagCFP cyan 458 / 480 64 %monomerfast
TagGFP green482 / 505 104 %monomerfast
TagYFP yellow508 / 524 120 %monomerfast
TagRFP red (orange)555 / 584 145 %monomerfast
TagFP635 far-red588 / 635 45 %monomerfast

TurboColors, superbright and fast-maturing proteins / details
TurboGFP green482 / 502 112 %dimersuper fast
TurboYFP yellow525 / 538 169 %dimersuper fast
TurboRFPred (orange)553 / 574 187 %dimersuper fast
TurboFP602 red574 / 602 79 %dimerfast
TurboFP635 far-red588 / 635 67 % dimersuper fast

Other available proteins
PhiYFP yellow525 / 537 158 %dimerfast
JRedred584 /  610 27 %dimerslow

Evaluation of the technologies is easily available by purchase of the related research use product. Please see "Notice to Purchaser" section for the product of your interest for details.

To facilitate evaluation of Evrogen fluorescent protein technology we offer custom assay development services including making fuses with the proteins of interest with following testing in cells (localization or translocation).

For license information please contact Evrogen by e-mail at license@evrogen.com.

References:

  • Chudakov DM, Lukyanov S, Lukyanov KA. Fluorescent proteins as a toolkit for in vivo imaging. Trends Biotechnol. 2005 Dec;23(12):605-13. / pmid: 16269193
  • Evdokimov AG, Pokross ME, Egorov NS, Zaraisky AG, Yampolsky IV, Merzlyak EM, Shkoporov AN, Sander I, Lukyanov KA, Chudakov DM. Structural basis for the fast maturation of Arthropoda green fluorescent protein. EMBO Rep. 2006; 7 (10):1006-12. / pmid: 16936637
  • Merzlyak EM, Goedhart J, Shcherbo D, Bulina ME, Shcheglov AS, Fradkov AF, Gaintzeva A, Lukyanov KA, Lukyanov S, Gadella TW, Chudakov DM. Bright monomeric red fluorescent protein with an extended fluorescence lifetime. Nat Methods. 2007; 4 (7):555-7. / pmid: 17572680
  • Shagin DA, Barsova EV, Yanushevich YG, Fradkov AF, Lukyanov KA, Labas YA, Semenova TN, Ugalde JA, Meyers A, Nunez JM, Widder EA, Lukyanov SA, Matz MV. GFP-like proteins as ubiquitous metazoan superfamily: evolution of functional features and structural complexity. Mol Biol Evol. 2004; 21 (5):841-50. / pmid: 14963095
  • Shcherbo D, Merzlyak EM, Chepurnykh TV, Fradkov AF, Ermakova GV, Solovieva EA, Lukyanov KA, Bogdanova EA, Zaraisky AG, Lukyanov S, Chudakov DM. Bright far-red fluorescent protein for whole-body imaging. Nat Methods. 2007; 4 (9):741-6. / pmid: 17721542
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