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    TurboGFP

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Green fluorescent protein TurboGFP

- Bright green fluorescence
- Fast maturation at a wide range of temperatures
- High pH-stability and photostability
- Proven suitability to generate stably transfected cell lines
- Destabilized variant is available
- Recommended for gene expression analysis and cell and organelle labeling

TurboGFP is an improved variant of the green fluorescent protein CopGFP cloned from copepod Pontellina plumata (Arthropoda; Crustacea; Maxillopoda; Copepoda) [Shagin et al., 2004]. It possesses bright green fluorescence (excitation/ emission max = 482/ 502 nm) that is visible earlier than fluorescence of other green fluorescent proteins.
TurboGFP is mainly intended for applications where fast appearance of bright fluorescence is crucial. It is specially recommended for cell and organelle labeling and tracking the promoter activity. Destabilized TurboGFP variant allows accurate analysis of rapid and/or transient events in gene regulation.

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The prices do not include delivery. The prices vary in different countries. Please contact your local distributor for exact prices and delivery information.


TurboGFP expression/source vectors

pTurboGFP-C FP511 Mammalian expression vector encoding humanized TurboGFP and allowing its expression and generation of fusions to the TurboGFP C-terminus 20 μg € 400
pTurboGFP-N FP512 Mammalian expression vector encoding humanized TurboGFP and allowing its expression and generation of fusions to the TurboGFP N-terminus 20 μg € 400
pTurboGFP-B FP513 Bacterial expression vector; source of the TurboGFP coding sequence 20 μg € 400
pTurboGFP-PRL FP515 Promoterless vector encoding humanized TurboGFP and designed for monitoring of activity of different promoters and promoter/enhancer combinations 20 μg € 400
pTurboGFP-mito FP517 Mammalian expression vector encoding humanized TurboGFP targeted to mitochondria 20 μg € 400
pTurboGFP-PRL-dest1 FP518 Promoterless vector encoding destabilized TurboGFP and designed for monitoring of activity of different promoters and promoter/enhancer combinations 20 μg € 400
pTurboGFP-dest1 FP519 Mammalian expression vector encoding destabilized TurboGFP for its expression and generation of fusions to the TurboGFP-dest1 N-terminus 20 μg € 400
Gateway® TurboGFP-C entry clone FP521 Gateway® entry clone for generation of fusions to the C-terminus of humanized TurboGFP; transfer of the constract encoding TurboGFP or its fusion into Gateway® destination vectors 20 μg € 400
Gateway® TurboGFP-N entry clone FP522 Gateway® entry clone for generation of fusions to the N-terminus of humanized TurboGFP; transfer of the constract encoding TurboGFP or its fusion into Gateway® destination vectors 20 μg € 400


Vector sets

Cell-Killer FPK01 pKillerRed-dMito vector encoding mitochondria-targeted KillerRed and pTurboGFP-N vector allowing cytoplasmic expression of bright green fluorescent protein TurboGFP for monitoring cell fate 20 μg each € 650
Membrane-Killer FPK02 pKillerRed-mem vector encoding membrane-targeted KillerRed and pTurboGFP-N vector allowing cytoplasmic expression of bright green fluorescent protein TurboGFP for monitoring cell fate 20 μg each € 650
Mito-tracker FPM01 Mammalian expression vectors for fluorescent labeling of mitochondria: pTurboGFP-mito, pPhi-Yellow-mito, and pKindling-Red-mito 20 μg each € 700
Promoter-tracker Green FPP03 Promoterless vectors pTurboGFP-PRL, pTurboGFP-PRL-dest1, and control vector pTurboGFP-dest1 20 μg each € 700
Promoter-tracker 3-colors FPP15 Promoterless vectors pTurboYFP-PRL, pTurboGFP-PRL, and pTurboRFP-PRL 20 μg each € 700


Recombinant protein

rTurboGFP FP552 Purified recombinant bright green fluorescent protein 100 μg € 130


Antibody against TurboGFP

Anti-TurboGFP(d)
antibody		 AB513 Rabbit polyclonal antibody against denatured TurboGFP and CopGFP 100 μg € 150
AB514 200 μg € 200

References:

  • Shagin DA, Barsova EV, Yanushevich YG, Fradkov AF, Lukyanov KA, Labas YA, Semenova TN, Ugalde JA, Meyers A, Nunez JM, Widder EA, Lukyanov SA, Matz MV. GFP-like proteins as ubiquitous metazoan superfamily: evolution of functional features and structural complexity. Mol Biol Evol. 2004; 21 (5):841-50. / pmid: 14963095
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