Basic fluorescent proteins

- Multicolor protein collection for in vivo labeling applications
- Direct expression in cells
- For research and commercial use

Evrogen offers a wide collection of bright basic fluorescent proteins (FPs) for applications in the field of live-cell assays including labeling of cells, subcellular structures, and proteins; analysis of promoter activity; generation of stably transfected cell lines expressing fluorescent proteins or their fusions.

Evrogen basic fluorescent proteins are divided into subgroups according to their properties and recommended applications:

TagFPs
Bright monomeric fluorescent proteins of different colors for protein localization and interaction studies

TurboFPs
Superbright and fast-maturing fluorescent proteins for gene expression analysis, cell and organelle labeling applications

NirFP
Near-infrared fluorescent protein NirFP for multicolor applications

Diversity of Evrogen basic fluorescent proteins

Ranging in color from blue to far-red, Evrogen fluorescent proteins can be used for multicolor labeling to observe different cellular events in a particular cell or a cell population.

TurboFPs excitation spectra. TurboFPs emission spectra.

Normalized excitation / emission spectra

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View in the Spectra viewer tool

List of fluorescent proteins
PRODUCT PAGE COLOR EX/EM MAX (nm) BRIGHTNESS,
% OF EGFP
STRUCTURE MATURATION RATE AT 37°C DESCRIPTION (PDF)
* Purified recombinant protein behaves as a pure monomer at concentrations of 10 mg/ml as verified by high-performance liquid chromatography (HPLC).
** Although NirFP is characterized by fast maturation in E. coli, due to low brightness it requires up to 48 hours for detection after transfection in mammalian cells.

TagFPs, protein localization tags / details
TagBFPblue402 / 45799monomerfast
TagCFPcyan458 / 48064monomerfast
TagGFP2green483 / 506105monomerfast
TagYFPyellow508 / 52494monomerfast
TagRFPred (orange)555 / 584148monomerfast
FusionRedred580 / 60853supermonomer*fast
mKate2far-red588 / 63374monomerfast

TurboFPs, superbright and fast-maturing proteins / details
TurboGFPgreen482 / 502112dimersuper fast
TurboYFPyellow525 / 538169dimersuper fast
TurboRFPred (orange)553 / 574187dimersuper fast
TurboFP602true-red574 / 60279dimerfast
TurboFP635far-red588 / 63567dimersuper fast
TurboFP650near-infrared592 / 65047dimersuper fast

Fluorescent proteins for special applications
NirFPnear-infrared605 / 67013dimerfast**

Old or discontinued variants
TagGFPgreen482 / 505104monomerfast
TagFP635far-red588 / 63545monomerfast
JRedtrue-red584 / 61026weak dimerslow
PhiYFPyellow525 / 537158dimerfast
CopGFPgreen482 / 502126tetramersuper fast
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Evrogen JSC, 16/10 Miklukho-Maklaya str., Moscow, Russia, Tel +7(495)988-4084, Fax +7(495)988-4085, e-mail:evrogen@evrogen.com