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    mKate2

Far-red fluorescent protein mKate2

- Super bright far-red fluorescence
- Monomeric protein with successful performance in fusions
- Fast maturation, high pH-stability and photostability, low cytotoxicity
- Proven suitability to generate stably transfected cell lines
- Fluorescent signal is easily distinguished from background fluorescence
- Recommended for protein labeling, multicolor applications and whole body imaging

Video of mKate2 fusions
in living cells
.

mKate2 is the next generation of far-red fluorescent protein TagFP635 (mKate) [Shcherbo et al., 2007; Shcherbo et al., 2009]. Possessing fluorescence with excitation/emission maxima at 588 and 633 nm, mKate2 is almost 3-fold brighter than TagFP635 and is 10-fold brighter than mPlum at physiological pH 7.5. Within the optical window optimal for light penetration in living tissues, calculated brightness of mKate2 is at least 2-fold higher compared to any monomeric fluorescent protein reported to date.

mKate2 is characterized by complete and fast chromophore maturation at 37°C with maturation half-time <20 min (versus 40 min for mCherry). It is more photostable under both widefield and confocal illumination than other monomeric far-red proteins, including TagFP635, mRaspberry and mPlum. The high brightness, far-red emission spectrum, excellent pH resistance and photostability, coupled with low toxicity demonstrated in transgenic Xenopus laevis embryos, make mKate2 a superior fluorescent tag for imaging in living tissues.

mKate2 is mainly intended for protein labeling. Its far-red fluorescence allows easy and reliable separation from standard green fluorescent labels in dual-color high-throughput assays.

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*50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.
The prices do not include delivery. The prices vary in different countries. Please contact your local distributor for exact prices and delivery information.


mKate2 expression/source vectors

pmKate2-C FP181 Mammalian expression vector encoding humanized mKate2 and allowing its expression and generation of fusions to the mKate2 C-terminus 20 μg € 400 / 200*
pmKate2-N FP182 Mammalian expression vector encoding humanized mKate2 and allowing its expression and generation of fusions to the mKate2 N-terminus 20 μg € 400 / 200*
pmKate2-actin FP184 Mammalian expression vector encoding humanized mKate2 fused with human cytoplasmic β-actin 20 μg € 400 / 200*
pmKate2-f-mem FP186 Mammalian expression vector encoding membrane-targeted mKate2 20 μg € 400 / 200*
pmKate2-mito FP187 Mammalian expression vector encoding humanized mKate2 targeted to mitochondria 20 μg € 400 / 200*
pmKate2-H2B FP311 Mammalian expression vector encoding humanized mKate2 fused with human histone H2B 20 μg € 400 / 200*
pmKate2-lyso FP312 Mammalian expression vector encoding humanized mKate2 targeted to lysosomes 20 μg € 400 / 200*
pmKate2-peroxi FP313 Mammalian expression vector encoding humanized mKate2 targeted to peroxisomes 20 μg € 400 / 200*
pmKate2-endo FP314 Mammalian expression vector encoding humanized mKate2 fused with human RhoB protein 20 μg € 400 / 200*
pmKate2-clathrin FP322 Mammalian expression vector encoding humanized mKate2 fused with human clathrin LCB 20 μg € 400 / 200*
pmKate2-ER FP324 Mammalian expression vector encoding humanized mKate2 targeted to the endoplasmic reticulum 20 μg € 400 / 200*


Antibody against mKate2

Anti-tRFP
antibody
AB233 Rabbit polyclonal antibody against TurboRFP, TurboFP602, TurboFP635, Katushka2S, TurboFP650, NirFP, TagBFP, TagRFP, FusionRed, TagFP635, mKate2, PA-TagRFP, mRuby and mCherry 100 μg € 220

References:

  • Shcherbo D, Merzlyak EM, Chepurnykh TV, Fradkov AF, Ermakova GV, Solovieva EA, Lukyanov KA, Bogdanova EA, Zaraisky AG, Lukyanov S, Chudakov DM. Bright far-red fluorescent protein for whole-body imaging. Nat Methods. 2007; 4 (9):741-6. / pmid: 17721542
  • Shcherbo D, Murphy CS, Ermakova GV, Solovieva EA, Chepurnykh TV, Shcheglov AS, Verkhusha VV, Pletnev VZ, Hazelwood KL, Roche PM, Lukyanov S, Zaraisky AG, Davidson MW, Chudakov DM. Far-red fluorescent tags for protein imaging in living tissues. Biochem J. 2009; 418 (3):567-74. doi: 10.1042/BJ20081949 / pmid: 19143658
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