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    mKate2

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Far-red fluorescent protein mKate2

- Super bright far-red fluorescence
- Monomeric protein with successful performance in fusions
- Fast maturation, high pH-stability and photostability
- Proven suitability to generate stably transfected cell lines
- Fluorescent signal is easily distinguished from background fluorescence
- Recommended for protein labeling, multicolor applications and whole body imaging

Video of mKate2 fusions
in living cells.

mKate2 is the next generation of far-red fluorescent protein TagFP635 (mKate) [Shcherbo et al., 2007; Shcherbo et al., 2009]. Possessing fluorescence with excitation/emission maxima at 588 and 633 nm, mKate2 is almost 3-fold brighter than TagFP635 and is 10-fold brighter than mPlum at physiological pH 7.5. Within the optical window optimal for light penetration in living tissues, calculated brightness of mKate2 is at least 2-fold higher compared to any monomeric fluorescent protein reported to date.

mKate2 is characterized by complete and fast chromophore maturation at 37°C with maturation half-time <20 min (versus 40 min for mCherry). It is more photostable under both widefield and confocal illumination than other monomeric far-red proteins, including TagFP635, mRaspberry and mPlum. The high brightness, far-red emission spectrum, excellent pH resistance and photostability, coupled with low toxicity demonstrated in transgenic Xenopus laevis embryos, make mKate2 a superior fluorescent tag for imaging in living tissues.

mKate2 is mainly intended for protein labeling. Its far-red fluorescence allows easy and reliable separation from standard green fluorescent labels in dual-color high-throughput assays.

detailed description

 
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mKate2 expression/source vectors

pmKate2-C FP181 Mammalian expression vector encoding humanized mKate2 and allowing its expression and generation of fusions to the mKate2 C-terminus 20 μg € 400
pmKate2-N FP182 Mammalian expression vector encoding humanized mKate2 and allowing its expression and generation of fusions to the mKate2 N-terminus 20 μg € 400
pmKate2-actin FP184 Mammalian expression vector encoding humanized mKate2 fused with human cytoplasmic β-actin 20 μg € 400
pmKate2-tubulin FP185 Mammalian expression vector encoding humanized mKate2 fused with human α-tubulin 20 μg € 400
pmKate2-f-mem FP186 Mammalian expression vector encoding membrane-targeted mKate2 20 μg € 400
pmKate2-mito FP187 Mammalian expression vector encoding humanized mKate2 targeted to mitochondria 20 μg € 400
pmKate2-laminB1 FP310 Mammalian expression vector encoding humanized mKate2 fused with human lamin B1 20 μg € 400
pmKate2-H2B FP311 Mammalian expression vector encoding humanized mKate2 fused with human histone H2B 20 μg € 400
pmKate2-lyso FP312 Mammalian expression vector encoding humanized mKate2 targeted to lysosomes 20 μg € 400
pmKate2-peroxi FP313 Mammalian expression vector encoding humanized mKate2 targeted to peroxisomes 20 μg € 400
pmKate2-endo FP314 Mammalian expression vector encoding humanized mKate2 fused with human RhoB protein 20 μg € 400
pmKate2-zyxin FP315 Mammalian expression vector encoding humanized mKate2 fused with human zyxin 20 μg € 400
pmKate2-EB3 FP316 Mammalian expression vector encoding humanized mKate2 fused with human EB3 protein 20 μg € 400
pmKate2-actinin FP317 Mammalian expression vector encoding humanized mKate2 fused with human α-actinin 20 μg € 400
pmKate2-vimentin FP318 Mammalian expression vector encoding humanized mKate2 fused with human vimentin 20 μg € 400
pmKate2-keratin FP319 Mammalian expression vector encoding humanized mKate2 fused with human cytokeratin-18 20 μg € 400
pmKate2-profilin FP320 Mammalian expression vector encoding humanized mKate2 fused with mouse profilin 20 μg € 400
pmKate2-annexin FP321 Mammalian expression vector encoding humanized mKate2 fused with human annexin 20 μg € 400
pmKate2-clathrin FP322 Mammalian expression vector encoding humanized mKate2 fused with human clathrin LCB 20 μg € 400
pmKate2-paxillin FP323 Mammalian expression vector encoding humanized mKate2 fused with chiken paxillin 20 μg € 400
pmKate2-ER FP324 Mammalian expression vector encoding humanized mKate2 targeted to the endoplasmic reticulum 20 μg € 400


Vector set

Fusion Far-Red FPF25 Mammalian expression vectors encoding mKate2 for its expression and fusion generation: pmKate2-N and pmKate2-C 20 μg each € 650


Antibody against mKate2

Anti-tRFP
antibody		 AB233 Rabbit polyclonal antibody against TurboRFP, TurboFP602, TurboFP635, TurboFP650, NirFP, TagBFP, TagRFP, TagFP635, mKate2 and PA-TagRFP 100 μg € 150
AB234 200 μg € 200

References:

  • Shcherbo D, Merzlyak EM, Chepurnykh TV, Fradkov AF, Ermakova GV, Solovieva EA, Lukyanov KA, Bogdanova EA, Zaraisky AG, Lukyanov S, Chudakov DM. Bright far-red fluorescent protein for whole-body imaging. Nat Methods. 2007; 4 (9):741-6. / pmid: 17721542
  • Shcherbo D, Murphy CS, Ermakova GV, Solovieva EA, Chepurnykh TV, Shcheglov AS, Verkhusha VV, Pletnev VZ, Hazelwood KL, Roche PM, Lukyanov S, Zaraisky AG, Davidson MW, Chudakov DM. Far-red fluorescent tags for protein imaging in living tissues. Biochem J. 2009; 418 (3):567-74. / pmid: 19143658
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