RESEARCH PRODUCTS/Basic fluorescent proteins/TagFPs:


    TagFP635

Far-red fluorescent protein TagFP635

- Bright far-red fluorescence
- Monomer with successful performance in fusions
- High photostability
- Fluorescent signal is easily distinguished from background fluorescence
- Recommended for protein labeling

TagFP635 (scientific name mKate) is a monomeric far-red fluorescent protein generated from the wild-type RFP from sea anemone Entacmaea quadricolor [Shcherbo et al., 2007]. It possesses bright fluorescence with excitation/emission maxima at 588 and 635 nm, respectively.
TagFP635 is 6.4-fold brighter than mPlum, 1.25-fold brighter and much more photostable than mRaspberry [Shcherbo et al., 2007; Shaner et al., 2004]. This makes it the best monomeric far-red fluorescent protein available so far.
TagFP635 is mainly intended for protein labeling. It fluorescence allows easy and reliable separation from standard green fluorescent labels in dual-color high-throughput assays. TagFP635 can also be used for cell and organelle labeling and for tracking the promoter activity; however, TurboFP635 is preferable for these applications as it is brighter and more pH-stable than TagFP635.

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TagFP635 expression/source vectors

pTagFP635-CFP161Mammalian expression vector encoding humanazied TagFP635 and allowing its expression and generation of fusions to the TagFP635 C-terminus20 μg€ 400.00
pTagFP635-NFP162Mammalian expression vector encoding humanazied TagFP635 and allowing its expression and generation of fusions to the TagFP635 N-terminus20 μg€ 400.00
pTagFP635-actininFP380Mammalian expression vector encoding humanazied TagFP635 fused with human α-actinin20 μg€ 400.00
pTagFP635-clathrinFP381Mammalian expression vector encoding humanazied TagFP635 fused with human clathrin light chain LCB20 μg€ 400.00
pTagFP635-Cx26FP382Mammalian expression vector encoding humanazied TagFP635 fused with rat connexin 2620 μg€ 400.00
pTagFP635-Cx32FP383Mammalian expression vector encoding humanazied TagFP635 fused with human connexin 3220 μg€ 400.00
pTagFP635-Cx43FP384Mammalian expression vector encoding humanazied TagFP635 fused with rat connexin 4320 μg€ 400.00
pTagFP635-EB3FP385Mammalian expression vector encoding humanazied TagFP635 fused with human EB3 protein20 μg€ 400.00
pTagFP635-H2BFP386Mammalian expression vector encoding humanazied TagFP635 fused with human histone H2B20 μg€ 400.00
pTagFP635-laminB1FP387Mammalian expression vector encoding humanazied TagFP635 fused with human lamin B120 μg€ 400.00
pTagFP635-vinculinFP388Mammalian expression vector encoding humanazied TagFP635 fused with human vinculin20 μg€ 400.00
pTagFP635-zyxinFP389Mammalian expression vector encoding humanazied TagFP635 fused with human zyxin20 μg€ 400.00
pTagFP635-actinFP390Mammalian expression vector encoding humanazied TagFP635 fused with human cytoplasmic β-actin20 μg€ 400.00
pTagFP635-tubulinFP391Mammalian expression vector encoding humanazied TagFP635 fused with human α-tubulin20 μg€ 400.00


Antibody against TagFP635

Anti-tRFP antibodyAB231Rabbit polyclonal antibody against TurboRFP, TurboFP602, TurboFP635, TagRFP, and TagFP635100 μg€ 150.00
AB232200 μg€ 200.00

References:

  • Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien RY. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol. 2004; 22 (12):1567-72. / pmid: 15558047
  • Shcherbo D, Merzlyak EM, Chepurnykh TV, Fradkov AF, Ermakova GV, Solovieva EA, Lukyanov KA, Bogdanova EA, Zaraisky AG, Lukyanov S, Chudakov DM. Bright far-red fluorescent protein for whole-body imaging. Nat Methods. 2007; 4 (9):741-6. / pmid: 17721542
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