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    TurboFP635

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Far-red fluorescent protein TurboFP635

- Super bright far-red fluorescence
- Fast maturation, high photostability
- Proven suitability to generate stably transfected cell lines
- Fluorescent signal is easily distinguished from background fluorescence
- Recommended for cell and organelle labeling in autofluorescent environment, multicolor applications and whole body imaging

TurboFP635 (scientific name Katushka) is a far-red mutant of the red fluorescent protein from sea anemone Entacmaea quadricolor [Shcherbo et al., 2007]. Possessing excitation/emission maxima at 588/635 nm, TurboFP635 is 7 to 10-fold brighter compared to the spectrally close HcRed [Gurskaya et al., 2001] or mPlum [Wang et al., 2004]. TurboFP635 is characterized by fast maturation and a high pH-stability and photostability. The unique characteristics of TurboFP635 make it the protein of choice for visualization within living tissues and dual-color high-throughput assays.

TurboFP635 is mainly intended for applications where fast appearance of far-red fluorescence is crucial. It is specially recommended for whole body imaging, cell and organelle labeling, and for tracking the promoter activity in auto-fluorescent tissues.

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TurboFP635 expression/source vectors

pTurboFP635-C FP721 Mammalian expression vector encoding humanized TurboFP635 and allowing its expression and generation of fusions to the TurboFP635 C-terminus 20 μg € 400
pTurboFP635-N FP722 Mammalian expression vector encoding humanized TurboFP635 and allowing its expression and generation of fusions to the TurboFP635 N-terminus 20 μg € 400


Antibody against TurboFP635

Anti-tRFP
antibody		 AB233 Rabbit polyclonal antibody against TurboRFP, TurboFP602, TurboFP635, TagBFP, TagRFP, TagFP635, and mKate2 100 μg € 150
AB234 200 μg € 200

References:

  • Gurskaya NG, Fradkov AF, Terskikh A, Matz MV, Labas YA, Martynov VI, Yanushevich YG, Lukyanov KA, Lukyanov SA. GFP-like chromoproteins as a source of far-red fluorescent proteins. FEBS Lett. 2001; 507 (1):16-20. / pmid: 11682051
  • Shcherbo D, Merzlyak EM, Chepurnykh TV, Fradkov AF, Ermakova GV, Solovieva EA, Lukyanov KA, Bogdanova EA, Zaraisky AG, Lukyanov S, Chudakov DM. Bright far-red fluorescent protein for whole-body imaging. Nat Methods. 2007; 4 (9):741-6. / pmid: 17721542
  • Wang L, Jackson WC, Steinbach PA, Tsien RY. Evolution of new nonantibody proteins via iterative somatic hypermutation. Proc Natl Acad Sci U S A. 2004; 101 (48):16745-9. / pmid: 15556995
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