TagRFP


Red fluorescent protein TagRFP

- Bright red (orange) fluorescence
- Fast maturation
- High pH stability
- Monomeric protein with successful performance in fusions
- Recommended for protein labeling

TagRFP is a monomeric red fluorescent protein generated from the wild-type RFP from sea anemone Entacmaea quadricolor [Merzlyak et al., 2007]. It possesses bright fluorescence with excitation/emission maxima at 555 and 584 nm, respectively. TagRFP is about three times brighter than mCherry protein [Shaner et al., 2004], which makes it the brightest monomeric red fluorescent protein available so far.
TagRFP is mainly intended for protein labeling. It can also be used for cell and organelle labeling and for tracking the promoter activity; however, TurboRFP is preferable for these applications as it is brighter and more photostable than TagRFP.

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TagRFP expression/source vectors

pTagRFP-CFP141Mammalian expression vector encoding humanazied TagRFP and allowing its expression and generation of fusions to the TagRFP C-terminus20 μg€ 400.00
pTagRFP-NFP142Mammalian expression vector encoding humanazied TagRFP and allowing its expression and generation of fusions to the TagRFP N-terminus20 μg€ 400.00
pTagRFP-actinFP144Mammalian expression vector encoding humanazied TagRFP fused with human cytoplasmic β-actin20 μg€ 400.00
pTagRFP-tubulinFP145Mammalian expression vector encoding humanazied TagRFP fused with human α-tubulin20 μg€ 400.00
pTagRFP-mitoFP147Mammalian expression vector encoding humanazied TagRFP targeted to mitochondria20 μg€ 400.00
pTagRFP-actininFP360Mammalian expression vector encoding humanazied TagRFP fused with human α-actinin20 μg€ 400.00
pTagRFP-integrinFP361Mammalian expression vector encoding humanazied TagRFP fused with human α-V-integrin20 μg€ 400.00
pTagRFP-Cx26FP362Mammalian expression vector encoding humanazied TagRFP fused with rat connexin 2620 μg€ 400.00
pTagRFP-Cx32FP363Mammalian expression vector encoding humanazied TagRFP fused with human connexin 3220 μg€ 400.00
pTagRFP-Cx43FP364Mammalian expression vector encoding humanazied TagRFP fused with rat connexin 4320 μg€ 400.00
pTagRFP-EB3FP365Mammalian expression vector encoding humanazied TagRFP fused with human EB3 protein20 μg€ 400.00
pTagRFP-FAKFP366Mammalian expression vector encoding humanazied TagRFP fused with chicken focal adhesion kinase20 μg€ 400.00
pTagRFP-GolgiFP367Mammalian expression vector encoding humanazied TagRFP fused with human Golgi targeting sequence (GTS)20 μg€ 400.00
pTagRFP-H2BFP368Mammalian expression vector encoding humanazied TagRFP fused with human histone H2B20 μg€ 400.00
pTagRFP-keratinFP369Mammalian expression vector encoding humanazied TagRFP fused with human cytokeratin-1820 μg€ 400.00
pTagRFP-laminB1FP370Mammalian expression vector encoding humanazied TagRFP fused with human lamin B120 μg€ 400.00
pTagRFP-profilinFP371Mammalian expression vector encoding humanazied TagRFP fused with mouse profilin20 μg€ 400.00
pTagRFP-vinculinFP372Mammalian expression vector encoding humanazied TagRFP fused with human vinculin20 μg€ 400.00
pTagRFP-zyxinFP373Mammalian expression vector encoding humanazied TagRFP fused with human zyxin20 μg€ 400.00


Vector set

Fusion RedFPF14Mammalian expression vectors encoding TagRFP for its expression and fusion generation: pTagRFP-C and pTagRFP-N20 μg each€ 650.00


Antibody against TagRFP

Anti-tRFP antibodyAB231Rabbit polyclonal antibody against TurboRFP, TurboFP602, TurboFP635, TagRFP, and TagFP635100 μg€ 150.00
AB232200 μg€ 200.00

References:

  • Merzlyak EM, Goedhart J, Shcherbo D, Bulina ME, Shcheglov AS, Fradkov AF, Gaintzeva A, Lukyanov KA, Lukyanov S, Gadella TW, Chudakov DM. Bright monomeric red fluorescent protein with an extended fluorescence lifetime. Nat Methods. 2007; 4 (7):555-7. / pmid: 17572680
  • Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien RY. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol. 2004; 22 (12):1567-72. / pmid: 15558047
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