RESEARCH PRODUCTS/Basic fluorescent proteins/TagFPs:

    TagRFP

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Red fluorescent protein TagRFP

- Bright red (orange) fluorescence
- Monomeric protein with successful performance in fusions
- Fast maturation, high pH-stability
- Proven suitability to generate stably transfected cell lines
- Recommended for protein labeling, acidic organelle labeling, FRET applications

TagRFP is a monomeric red (orange) fluorescent protein generated from the wild-type RFP from sea anemone Entacmaea quadricolor [Merzlyak et al., 2007]. It possesses bright fluorescence with excitation/emission maxima at 555 and 584 nm, respectively. TagRFP is about three times brighter than mCherry protein [Shaner et al., 2004], which makes it the brightest monomeric red fluorescent protein available so far.
TagRFP is mainly intended for protein labeling and FRET applications. It can also be used for cell and organelle labeling and for tracking the promoter activity.

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TagRFP expression/source vectors

pTagRFP-C FP141 Mammalian expression vector encoding humanized TagRFP and allowing its expression and generation of fusions to the TagRFP C-terminus 20 μg € 400
pTagRFP-N FP142 Mammalian expression vector encoding humanized TagRFP and allowing its expression and generation of fusions to the TagRFP N-terminus 20 μg € 400
pTagRFP-actin FP144 Mammalian expression vector encoding humanized TagRFP fused with human cytoplasmic β-actin 20 μg € 400
pTagRFP-tubulin FP145 Mammalian expression vector encoding humanized TagRFP fused with human α-tubulin 20 μg € 400
pTagRFP-mito FP147 Mammalian expression vector encoding humanized TagRFP targeted to mitochondria 20 μg € 400
Gateway® TagRFP-AS-C entry clone FP148 Gateway® entry clone for generation of fusions to the C-terminus of TagRFP; transfer of the constract encoding TagRFP or its fusion into Gateway® destination vectors; TagRFP codon usage is optimized for expression in Arabidopsis and Saccharomyces 20 μg € 400
Gateway® TagRFP-AS-N entry clone FP149 Gateway® entry clone for generation of fusions to the N-terminus of TagRFP; transfer of the constract encoding TagRFP or its fusion into Gateway® destination vectors; TagRFP codon usage is optimized for expression in Arabidopsis and Saccharomyces 20 μg € 400
pTagRFP-actinin FP360 Mammalian expression vector encoding humanized TagRFP fused with human α-actinin 20 μg € 400
pTagRFP-integrin FP361 Mammalian expression vector encoding humanized TagRFP fused with human α-V-integrin 20 μg € 400
pTagRFP-Cx26 FP362 Mammalian expression vector encoding humanized TagRFP fused with rat connexin 26 20 μg € 400
pTagRFP-Cx32 FP363 Mammalian expression vector encoding humanized TagRFP fused with human connexin 32 20 μg € 400
pTagRFP-Cx43 FP364 Mammalian expression vector encoding humanized TagRFP fused with rat connexin 43 20 μg € 400
pTagRFP-EB3 FP365 Mammalian expression vector encoding humanized TagRFP fused with human EB3 protein 20 μg € 400
pTagRFP-FAK FP366 Mammalian expression vector encoding humanized TagRFP fused with chicken focal adhesion kinase 20 μg € 400
pTagRFP-Golgi FP367 Mammalian expression vector encoding humanized TagRFP fused with human Golgi targeting sequence (GTS) 20 μg € 400
pTagRFP-H2B FP368 Mammalian expression vector encoding humanized TagRFP fused with human histone H2B 20 μg € 400
pTagRFP-keratin FP369 Mammalian expression vector encoding humanized TagRFP fused with human cytokeratin-18 20 μg € 400
pTagRFP-laminB1 FP370 Mammalian expression vector encoding humanized TagRFP fused with human lamin B1 20 μg € 400
pTagRFP-profilin FP371 Mammalian expression vector encoding humanized TagRFP fused with mouse profilin 20 μg € 400
pTagRFP-vinculin FP372 Mammalian expression vector encoding humanized TagRFP fused with human vinculin 20 μg € 400
pTagRFP-zyxin FP373 Mammalian expression vector encoding humanized TagRFP fused with human zyxin 20 μg € 400


Vector set

Fusion Red FPF14 Mammalian expression vectors encoding TagRFP for its expression and fusion generation: pTagRFP-C and pTagRFP-N 20 μg each € 650


Antibody against TagRFP

Anti-tRFP
antibody		 AB233 Rabbit polyclonal antibody against TurboRFP, TurboFP602, TurboFP635, TagBFP, TagRFP, TagFP635 and mKate2 100 μg € 150
AB234 200 μg € 200

References:

  • Merzlyak EM, Goedhart J, Shcherbo D, Bulina ME, Shcheglov AS, Fradkov AF, Gaintzeva A, Lukyanov KA, Lukyanov S, Gadella TW, Chudakov DM. Bright monomeric red fluorescent protein with an extended fluorescence lifetime. Nat Methods. 2007; 4 (7):555-7. / pmid: 17572680
  • Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien RY. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol. 2004; 22 (12):1567-72. / pmid: 15558047
  • Shcherbo D, Souslova EA, Goedhart J, Chepurnykh TV, Gaintzeva A, Shemiakina II, Gadella TW, Lukyanov S, Chudakov DM. Practical and reliable FRET/FLIM pair of fluorescent proteins. BMC Biotechnol. 2009; 9 :24. / pmid: 19321010
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