TurboYFP


Yellow fluorescent protein TurboYFP

- Superbright true-yellow fluorescence
- Fast maturation
- Emission wavelength is ideally positioned between those of green and red fluorescent proteins
- Destabilized version is available
- Recommended for gene expression analysis, cell and organelle labeling

TurboYFP is an enhanced variant of the yellow fluorescent protein PhiYFP from jellyfish Phialidium sp. [Shagin et al., 2004]. Possessing superbright yellow fluorescence with emission maximum at 538 nm, TurboYFP is ideally positioned between green and red fluorescent proteins, allowing easy separation of these fluorescent markers by flow cytometry using common channels of detection and a single laser excitation line. Compared with Phi-Yellow proteins, TurboYFP matures faster in mammalian cells.
TurboYFP is mainly intended for applications where fast appearance of bright fluorescence is crucial. It is specially recommended for cell labeling and tracking the promoter activity. Destabilized TurboYFP variant allows accurate analysis of rapid and/or transient events in gene regulation.

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TurboYFP expression/source vectors

pTurboYFP-CFP611Mammalian expression vector encoding humanazied TurboYFP and allowing its expression and generation of fusions to the TurboYFP C-terminus20 μg€ 400.00
pTurboYFP-NFP612Mammalian expression vector encoding humanazied TurboYFP and allowing its expression and generation of fusions to the TurboYFP N-terminus20 μg€ 400.00
pTurboYFP-BFP613Bacterial expression vector; source of the TurboYFP coding sequence20 μg€ 400.00
pTurboYFP-PRLFP615Promoterless vector encoding humanazied TurboYFP and designed for monitoring transcription from different promoters and promoter/enhancer combinations20 μg€ 400.00
pTurboYFP-PRL-dest1FP618Promoterless vector encoding destabilized TurboYFP and designed for monitoring transcription from different promoters and promoter/enhancer combinations20 μg€ 400.00
pTurboYFP-dest1FP619Mammalian expression vector encoding destabilized TurboYFP for its expression and generation of fusions to the TurboYFP-dest1 N-terminus20 μg€ 400.00


Vector sets

Promoter-tracker YellowFPP14Promoterless vectors pTurboYFP-PRL, pTurboYFP-PRL-dest1, and control vector pTurboYFP-dest120 μg each€ 700.00
Promoter-tracker 3-colorsFPP15Promoterless vectors pTurboYFP-PRL, pTurboGFP-PRL, and pTurboRFP-PRL20 μg each€ 700.00


Antibodies against TurboYFP

Anti-PhiYFP antibodyAB601Rabbit polyclonal antibody against non-denatured PhiYFP, PhiYFP-m, and TurboYFP100 μg€ 150.00
AB602200 μg€ 200.00
Anti-PhiYFP(d) antibodyAB603Rabbit polyclonal antibody against denatured PhiYFP, PhiYFP-m, and TurboYFP100 μg€ 150.00
AB604200 μg€ 200.00

References:

  • Shagin DA, Barsova EV, Yanushevich YG, Fradkov AF, Lukyanov KA, Labas YA, Semenova TN, Ugalde JA, Meyers A, Nunez JM, Widder EA, Lukyanov SA, Matz MV. GFP-like proteins as ubiquitous metazoan superfamily: evolution of functional features and structural complexity. Mol Biol Evol. 2004; 21 (5):841-50. / pmid: 14963095
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