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Phi-Yellow fluorescent proteins
- Bright yellow fluorescence
- Proven suitability to generate stably transfected cell lines
- Destabilized variant is available
 | PhiYFP and PhiYFP-m are the mutants of a natural yellow fluorescent protein from Phialidium sp. (Cnidaria; Hydrozoa; Hydroida; Leptomedusae; Campanulariidae)[Shagin et al., 2004] and previous versions of TurboYFP.
Excitation/emission maxima of Phi-Yellow proteins are at 482 and 505 nm, respectively. They exhibit less brightness and maturation rate than TurboYFP, but are more suitable for generation of stably transfected cell lines. The emission wavelength of these proteins is ideally positioned between those of green and red fluorescent proteins, allowing easy separation of these fluorescent tags by flow cytometry using common channels of detection and a single laser excitation line. |
Related products| PRODUCT | CAT.# | DESCRIPTION | SIZE | PRICE | | The prices do not include delivery. The prices vary in different countries. Please contact your local distributor for exact prices and delivery information. |
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| PhiYFP expression/source vectors |
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| pPhi-Yellow-C | FP601 | Mammalian expression vector encoding humanazied PhiYFP-m and allowing its expression and generation of fusions to the PhiYFP-m C-terminus | 20 μg | € 400.00 | | pPhi-Yellow-N | FP602 | Mammalian expression vector encoding humanazied PhiYFP and allowing its expression and generation of fusions to the PhiYFP N-terminus | 20 μg | € 400.00 | | pPhi-Yellow-B | FP603 | Bacterial expression vector; source of the PhiYFP coding sequence | 20 μg | € 400.00 | | pPhi-Yellow-PRL | FP604 | Promoterless vector encoding humanazied PhiYFP and designed for monitoring transcription from different promoters and promoter/enhancer combinations | 20 μg | € 400.00 | | pPhi-Yellow-PRL-dest1 | FP605 | Promoterless vector encoding destabilized PhiYFP-m and designed for monitoring transcription from different promoters and promoter/enhancer combinations | 20 μg | € 400.00 | | pPhi-Yellow-peroxi | FP606 | Mammalian expression vector encoding humanazied PhiYFP-m targeted to peroxisomes | 20 μg | € 400.00 | | pPhi-Yellow-mito | FP607 | Mammalian expression vector encoding humanazied PhiYFP targeted to mitochondria | 20 μg | € 400.00 | | pPhi-Yellow-dest1 | FP608 | Mammalian expression vector encoding destabilized PhiYFP-m for its expression and generation of fusions to the PhiYFP-m-dest1 N-terminus | 20 μg | € 400.00 |
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| Vector set |
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| Mito-tracker | FPM01 | Mammalian expression vectors for fluorescent labeling of mitochondria: pTurboGFP-mito, pPhi-Yellow-mito, and pKindling-Red-mito | 20 μg each | € 700.00 |
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| Recombinant protein |
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| rPhiYFP | FP651 | Purified recombinant bright yellow fluorescent protein | 100 μg | € 130.00 |
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| Antibodies against PhiYFP |
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| Anti-PhiYFP antibody | AB601 | Rabbit polyclonal antibody against non-denatured PhiYFP, PhiYFP-m, and TurboYFP | 100 μg | € 150.00 | | AB602 | 200 μg | € 200.00 | | Anti-PhiYFP(d) antibody | AB603 | Rabbit polyclonal antibody against denatured PhiYFP, PhiYFP-m, and TurboYFP | 100 μg | € 150.00 | | AB604 | 200 μg | € 200.00 |
References:
- Shagin DA, Barsova EV, Yanushevich YG, Fradkov AF, Lukyanov KA, Labas YA, Semenova TN, Ugalde JA, Meyers A, Nunez JM, Widder EA, Lukyanov SA, Matz MV. GFP-like proteins as ubiquitous metazoan superfamily: evolution of functional features and structural complexity. Mol Biol Evol. 2004; 21 (5):841-50. / pmid: 14963095
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