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![]() TurboYFP
SUPPORTRESOURCES |
Yellow fluorescent protein TurboYFP- Superbright true-yellow fluorescence
Main properties
Recommended filter sets and antibodiesTurboYFP can be recognized using Anti-PhiYFP (Cat.# AB601-AB602) and Anti-PhiYFP(d) (Cat.# AB603-AB604) antibodies available from Evrogen. TurboYFP can be detected using Omega Optical filter set XF104-3 or Chroma Technology Corp. filter set 42003 ("ZsYellow1"). Performance and useTurboYFP can be expressed and detected in a wide range of organisms. Mammalian cells transiently transfected with TurboYFP expression vectors give bright fluorescent signals within 8-10 hrs after transfection. Being overexpressed in long-term culture of cells with high expression levels, TurboYFP shows slight tendency to aggregate. It might limit TurboYFP use in such experimental systems. Please use Phi-Yellow proteins for stable expression and for organelle labeling. TurboYFP can be expressed and detected in a wide range of organisms. Mammalian cells transiently transfected with TurboYFP expression vectors give bright fluorescent signals within 8-10 hrs after transfection. No cell toxic effects are observed. TurboYFP can be used in multicolor labeling applications with cyan, green, red, and far-red fluorescent dyes.
Available variants and fusionsTurboYFP codon usage is optimized for high expression in mammalian cells [Haas et al., 1996], but it can be successfully expressed in many other heterological systems. Destabilized TurboYFP variant (TurboYFP-dest1): TurboYFP-dest1 is produced by fusing the initial protein with PEST amino acid sequence encoded by region 422-461 of mouse ornithine decarboxylase gene [Li et al., 1998]. This sequence targets the protein to degradation and enables a rapid protein turnover. TurboYFP-dest1 retains spectral properties of the initial protein, but has shorter half-lives (approximately 1.5-2 hrs) as measured by the analysis of fluorescence intensity of cells treated with a protein synthesis inhibitor, cycloheximide. Because of rapid turnover, TurboYFP-dest1 can be used to measure changes in gene expression. Phi-Yellow proteins: PhiYFP and PhiYFP-m are previous variants of TurboYFP. They possesses same exitation and emission spectra but have a few lesser maturation rate in mammalian cells. Phi-Yellow proteins do not aggregate in long-term cell cultures and in fusions with subcellular localization signals. They have proven suitability to generate stably transfected cell lines. References:
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