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Yellow fluorescent protein TurboYFP
- Super bright true-yellow fluorescence
- Emission wavelength is ideally positioned between those of green and red fluorescent proteins
- Fast maturation, high pH-stability and photostability
- Destabilized variant is available
- Recommended for gene expression analysis and cell labeling
Available variants and fusions
| Variant | Description | Related vector | Cat.# |
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Humanized TurboYFP
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TurboYFP codon usage is optimized for high expression in mammalian cells [Haas et al., 1996], but it can be successfully expressed in many other heterological systems.
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pTurboYFP-N
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FP612
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pTurboYFP-B
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FP613
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Destabilized TurboYFP variant (TurboYFP-dest1)
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TurboYFP-dest1 is produced by fusing the initial protein with PEST amino acid sequence encoded by region 422-461 of mouse ornithine decarboxylase gene [Li et al., 1998]. This sequence targets the protein to degradation and enables a rapid protein turnover. TurboYFP-dest1 retains spectral properties of the initial protein, but has shorter half-life (approximately 2 hrs) as measured by the analysis of fluorescence intensity of cells treated with a protein synthesis inhibitor, cycloheximide. Because of rapid turnover, TurboYFP-dest1 can be used to measure changes in gene expression.
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peTurboYFP-dest1
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FP617
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References:
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Haas J, Park EC, Seed B.
Codon usage limitation in the expression of HIV-1 envelope glycoprotein.
Curr Biol. 1996; 6 (3):315-24. / pmid: 8805248
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Li X, Zhao X, Fang Y, Jiang X, Duong T, Fan C, Huang CC, Kain SR.
Generation of destabilized green fluorescent protein as a transcription reporter.
J Biol Chem. 1998; 273 (52):34970-5. / pmid: 9857028
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